In situ synthesized nanozyme for photoacoustic-imaging-guided photothermal therapy and tumor hypoxia relief
Chaoyi Chen,
Yuwen Chen,
Xuanhao Wang,
Lulu Zhang,
Yan Luo,
Qingshuang Tang,
Yuan Wang,
Xiaolong Liang,
Cheng Ma
Affiliations
Chaoyi Chen
Department of Electronic Engineering, Beijing National Research Center for Information Science and Technology, Tsinghua University, Beijing 100084, China
Yuwen Chen
Department of Electronic Engineering, Beijing National Research Center for Information Science and Technology, Tsinghua University, Beijing 100084, China
Xuanhao Wang
Department of Electronic Engineering, Beijing National Research Center for Information Science and Technology, Tsinghua University, Beijing 100084, China
Lulu Zhang
Department of Ultrasound, Peking University Third Hospital, Beijing 100191, China
Yan Luo
Department of Electronic Engineering, Beijing National Research Center for Information Science and Technology, Tsinghua University, Beijing 100084, China
Qingshuang Tang
Department of Ultrasound, Peking University Third Hospital, Beijing 100191, China
Yuan Wang
Department of Ultrasound, Peking University Third Hospital, Beijing 100191, China
Xiaolong Liang
Department of Ultrasound, Peking University Third Hospital, Beijing 100191, China; Corresponding author
Cheng Ma
Department of Electronic Engineering, Beijing National Research Center for Information Science and Technology, Tsinghua University, Beijing 100084, China; Institute for Precision Healthcare, Tsinghua University, Beijing 100084, China; Corresponding author
Summary: Nanozymes have attracted extensive research interest due to their ideal enzymatic catalytic performance; however, uncontrollable activities and nonspecific accumulation limit their further clinical application. To overcome these obstacles, we proposed in situ synthesized nanozyme, and realized the concept through an intelligent nanosystem (ISSzyme) based on Prussian blue (PB) precursor. PB nanozyme was synthesized at the tumor sites through the interaction of ISSzyme with glutathione, which was demonstrated by comparing with conventional PB nanozyme. ISSzyme is capable of tumor-specific photoacoustic imaging (PAI) and photothermal therapy (PTT), reducing the false-positive signals of PAI and the treatment side effects of PTT. ISSzyme has catalase-like activities, resulting in tumor hypoxia relief and metastasis inhibition. More importantly, the in situ synthesized PB nanozyme has the favorable property of minimal liver accumulation. Considering the above advantages, ISSzyme is expected to shed light on the design of the next-generation artificial enzymes, with many new biomedical applications.
