Infection Ecology & Epidemiology (Nov 2015)

Serologic screening for 13 infectious agents in roe deer (Capreolus capreolus) in Flanders

  • Paul Tavernier,
  • Stanislas U. Sys,
  • Kris De Clercq,
  • Ilse De Leeuw,
  • Anne Brigitte Caij,
  • Miet De Baere,
  • Nick De Regge,
  • David Fretin,
  • Virginie Roupie,
  • Marc Govaerts,
  • Paul Heyman,
  • Daisy Vanrompay,
  • Lizi Yin,
  • Isabelle Kalmar,
  • Vanessa Suin,
  • Bernard Brochier,
  • Alexandre Dobly,
  • Stéphane De Craeye,
  • Sophie Roelandt,
  • Els Goossens,
  • Stefan Roels

DOI
https://doi.org/10.3402/iee.v5.29862
Journal volume & issue
Vol. 5, no. 0
pp. 1 – 12

Abstract

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Introduction: In order to investigate the role of roe deer in the maintenance and transmission of infectious animal and human diseases in Flanders, we conducted a serologic screening in 12 hunting areas. Materials and methods: Roe deer sera collected between 2008 and 2013 (n=190) were examined for antibodies against 13 infectious agents, using indirect enzyme-linked immunosorbent assay, virus neutralisation, immunofluorescence, or microagglutination test, depending on the agent. Results and discussion: High numbers of seropositives were found for Anaplasma phagocytophilum (45.8%), Toxoplasma gondii (43.2%) and Schmallenberg virus (27.9%), the latter with a distinct temporal distribution pattern following the outbreak in domestic ruminants. Lower antibody prevalence was found for Chlamydia abortus (6.7%), tick-borne encephalitis virus (5.1%), Neospora caninum (4.8%), and Mycobacterium avium subsp paratuberculosis (4.1%). The lowest prevalences were found for Leptospira (1.7%), bovine viral diarrhoea virus 1 (1.3%), and Coxiella burnetii (1.2%). No antibodies were found against Brucella sp., bovine herpesvirus 1, and bluetongue virus. A significant difference in seroprevalence between ages (higher in adults >1 year) was found for N. caninum. Four doubtful reacting sera accounted for a significant difference in seroprevalence between sexes for C. abortus (higher in females). Conclusions: Despite the more intensive landscape use in Flanders, the results are consistent with other European studies. Apart from maintaining C. abortus and MAP, roe deer do not seem to play an important role in the epidemiology of the examined zoonotic and domestic animal pathogens. Nevertheless, their meaning as sentinels should not be neglected in the absence of other wild cervid species.

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