Electroanalysis of Naringin at Electroactivated Pencil Graphite Electrode for the Assessment of Polyphenolics with Intermediate Antioxidant Power
Iulia Gabriela David,
Simona Carmen Litescu,
Raluca Moraru,
Camelia Albu,
Mihaela Buleandra,
Dana Elena Popa,
Sorin Riga,
Adela Magdalena Ciobanu,
Hassan Noor
Affiliations
Iulia Gabriela David
Department of Analytical Chemistry and Physical Chemistry, Faculty of Chemistry, University of Bucharest, Panduri Av. 90-92, District 5, 050663 Bucharest, Romania
Simona Carmen Litescu
National Institute of Research and Development for Biological Sciences, 296 Independenței Bd., District 6, 060031 Bucharest, Romania
Raluca Moraru
Department of Analytical Chemistry and Physical Chemistry, Faculty of Chemistry, University of Bucharest, Panduri Av. 90-92, District 5, 050663 Bucharest, Romania
Camelia Albu
National Institute of Research and Development for Biological Sciences, 296 Independenței Bd., District 6, 060031 Bucharest, Romania
Mihaela Buleandra
Department of Analytical Chemistry and Physical Chemistry, Faculty of Chemistry, University of Bucharest, Panduri Av. 90-92, District 5, 050663 Bucharest, Romania
Dana Elena Popa
Department of Analytical Chemistry and Physical Chemistry, Faculty of Chemistry, University of Bucharest, Panduri Av. 90-92, District 5, 050663 Bucharest, Romania
Sorin Riga
Department of Stress Research & Prophylaxis “Prof. Dr. Al. Obregia” Clinical Hospital of Psychiatry, Berceni Av. 10, District 4, 041914 Bucharest, Romania
Adela Magdalena Ciobanu
Department of Psychiatry “Prof. Dr. Al. Obregia” Clinical Hospital of Psychiatry, Berceni Av. 10, District 4, 041914 Bucharest, Romania
Hassan Noor
Department of Surgery, Faculty of Medicine, “Lucian Blaga” University Sibiu, Lucian Blaga Street 25, 550169 Sibiu, Romania
A simple and rapid differential pulse voltammetric (DPV) method using a single-use electroactivated pencil graphite electrode (PGE*) is proposed for the rapid screening of the total content of polyphenolics (TCP) with intermediate antioxidant power (AOP) in grapefruit peel and fresh juice. The results were compared and correlated with those provided by the HPLC-DAD-MS method. NG voltammetric behavior at PGE* was studied by cyclic voltammetry and an oxidation mechanism was suggested. The experimental conditions (type of PGE, electroactivation procedure, pH, nature and concentration of supporting electrolyte) for NG DPV determination were optimized. The NG peak current varied linearly with the concentration in the ranges 1.40 × 10−6–2.00 × 10−5 and 2.00 × 10−5–1.40 × 10−4 mol/L NG and a limit of detection (LoD) of 6.02 × 10−7 mol/L NG was attained. The method repeatability expressed as relative standard deviation was 7.62% for the concentration level of 2.00 × 10−6 mol/L NG. After accumulation for 240 s of NG at PGE* the LoD was lowered to 1.35 × 10−7 mol/L NG, the linear range being 6.00 × 10−7–8.00 × 10−6 mol/L NG. The developed electrochemical system was successfully tested on real samples and proved to be a cost-effective tool for the simple estimation of the TCP with intermediate AOP in citrus fruits.
