Česká Stomatologie a Praktické Zubní Lékařství (Jun 2012)

Effect of Macleaya Cordata (Willd) R.Br. Extract on Expression of Inflammatory Markers and Oxidative Stress in Gingival Fibroblasts

  • K. Chytilová,
  • A. Galandáková,
  • J. Pazdera,
  • A. Rajnochová Svobodová,
  • V. Šimánek

DOI
https://doi.org/10.51479/cspzl.2012.008
Journal volume & issue
Vol. 112, no. 2
pp. 47 – 56

Abstract

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Introduction: Macleaya cordata (Willd.) contains quaternary benzo[c]phenantridine alkaloids sanguinarine and chelerythrine. The alkaloid extract of M. cordata (MCE) is known for its anti-microbial and anti-inflammatory activities and thus is used as an active component in dental care products. Aim of work: The study examined the effects of MCE on lipopolysaccharide-induced inflammation and oxidative stress in human gingival fibroblasts. Material and Methods: The effect of MCE (0.01-10 µg/ml) on human gingival fibroblasts viability was evaluated using neutral red incorporation in the cells. Non-toxic concentrations of the extract (0.25 and 0.5 µg/ml) were used for evaluation of MCE effects on GSH level (GSH), reactive oxygen species (ROS) generation, cyclooxygenase-2 (COX-2) and interleukine-6 (IL-6) expression in cells treated with lipopolysaccharide (LPS). Results: MCE components induced the increase in intracellular GSH, decrease in ROS generation and reduction of the IL-6 and COX-6 expression in LPS-treated human gingival fibroblasts. Conclusion: The alkaloid extract of M. cordata suppresses the inflammation and oxidative stress in human gingival fibroblasts and it could be practically used in the treatment of periodontitis and alveolitis.

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