Functional genetic encoding of sulfotyrosine in mammalian cells

Xinyuan He; Yan Chen; Daisy Guiza Beltran; Maia Kelly; Bin Ma; Justin Lawrie; Feng Wang; Eric Dodds; Limei Zhang; Jiantao Guo; Wei Niu

doi:10.1038/s41467-020-18629-9

Nature Communications (Sep 2020)

Functional genetic encoding of sulfotyrosine in mammalian cells

Xinyuan He,
Yan Chen,
Daisy Guiza Beltran,
Maia Kelly,
Bin Ma,
Justin Lawrie,
Feng Wang,
Eric Dodds,
Limei Zhang,
Jiantao Guo,
Wei Niu

Affiliations

Xinyuan He: Department of Chemical & Biomolecular Engineering, University of Nebraska-Lincoln
Yan Chen: Department of Chemistry, University of Nebraska-Lincoln
Daisy Guiza Beltran: Department of Biochemistry, University of Nebraska-Lincoln
Maia Kelly: Department of Chemistry, University of Nebraska-Lincoln
Bin Ma: Department of Chemistry, University of Nebraska-Lincoln
Justin Lawrie: Department of Chemistry, University of Nebraska-Lincoln
Feng Wang: Institute of Biophysics, Chinese Academy of Sciences
Eric Dodds: Department of Chemistry, University of Nebraska-Lincoln
Limei Zhang: Department of Biochemistry, University of Nebraska-Lincoln
Jiantao Guo: Department of Chemistry, University of Nebraska-Lincoln
Wei Niu: Department of Chemical & Biomolecular Engineering, University of Nebraska-Lincoln

DOI: https://doi.org/10.1038/s41467-020-18629-9
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 10

Abstract

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Protein tyrosine O-sulfation is crucial for biomolecular interactions. Here the authors report in vitro engineering and in vivo validation of a tyrosyl-tRNA synthetase mutant for the genetic encoding of sulfotyrosine in mammalian cells.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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