Direct observation of DNA target searching and cleavage by CRISPR-Cas12a

Yongmoon Jeon; You Hee Choi; Yunsu Jang; Jihyeon Yu; Jiyoung Goo; Gyejun Lee; You Kyeong Jeong; Seung Hwan Lee; In-San Kim; Jin-Soo Kim; Cherlhyun Jeong; Sanghwa Lee; Sangsu Bae

doi:10.1038/s41467-018-05245-x

Nature Communications (Jul 2018)

Direct observation of DNA target searching and cleavage by CRISPR-Cas12a

Yongmoon Jeon,
You Hee Choi,
Yunsu Jang,
Jihyeon Yu,
Jiyoung Goo,
Gyejun Lee,
You Kyeong Jeong,
Seung Hwan Lee,
In-San Kim,
Jin-Soo Kim,
Cherlhyun Jeong,
Sanghwa Lee,
Sangsu Bae

Affiliations

Yongmoon Jeon: Center for Theragnosis, Korea Institute of Science and Technology
You Hee Choi: Advanced Photonics Research Institute, Gwangju Institute of Science and Technology
Yunsu Jang: Advanced Photonics Research Institute, Gwangju Institute of Science and Technology
Jihyeon Yu: Department of Chemistry, Hanyang University
Jiyoung Goo: Center for Theragnosis, Korea Institute of Science and Technology
Gyejun Lee: Center for Theragnosis, Korea Institute of Science and Technology
You Kyeong Jeong: Department of Chemistry, Hanyang University
Seung Hwan Lee: Center for Genome Engineering, Institute for Basic Science
In-San Kim: Center for Theragnosis, Korea Institute of Science and Technology
Jin-Soo Kim: Center for Genome Engineering, Institute for Basic Science
Cherlhyun Jeong: Center for Theragnosis, Korea Institute of Science and Technology
Sanghwa Lee: Advanced Photonics Research Institute, Gwangju Institute of Science and Technology
Sangsu Bae: Department of Chemistry, Hanyang University

DOI: https://doi.org/10.1038/s41467-018-05245-x
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 11

Abstract

Read online

Cas12a is a RNA-guided DNA endonuclease whose detailed mechanisms of target searching and DNA cleavage remained unclear. Here authors use single-molecule fluorescence assays to show that Cas12a searches for their on-target site.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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