Age related neoplasm in experimental animals

Abstract

The albino rats are commonly recognized as tumor-resistant and appreciable research interest because of the scarcity of reports and article papers on neoplasm tumors. In this regard, we have encountered ten male and female spontaneous cases of tumor investigated in the veterinary medicine college, experimental animal house. Liver and kidney neoplasms were observed in the animals, ages 2-2.5 years. Tumor masses were measured grossly, giving different shapes and sizes. Histopathological analyses of these tumors revealed degenerative changes represented by cystic alteration, basophilic cell infiltration, reduced cytoplasm, permanent hyperchromatic nuclei, necrosis mass, papillary projection, metaplasia of epithelial lining, nest of cancerous cell, and tubules of tumors cell, in addition to clumpy chromatin with irregular nuclear membranes as well. The neoplastic cell had a positive immunoreactive. The correlation coefficient between BCL2&PAX8 showed significant differences; IHC scoring was done based on the observations of cell reactivity in each field as follows: negative and positive represented by weak reaction as five cytoplasmic positive cells per field, moderate IHC reactivity was 10-15cells positive, furthermore, intense positive staining where more than 15 cytoplasmic positively cell per field. Our results suggest that spontaneous aged-related neoplasms represented by renal and hepatocellular carcinoma were at stages 3 and 4 in the liver and kidney. Our study's conclusion revealed numerous markers of primary hepatic and renal cell carcinoma, yet a specific marker cannot be used to identify all types of cancer. The study and investigation of these markers will significantly impact the early detection, prognosis, and treatment of cancer.

Keywords

• albino
• pax8
• bcl2
• necrosis