Juvenis Scientia (Apr 2021)
ANALYSIS OF THE FREQUENCY OF DETECTION OF ALLELES AND GENOTYPES OF POLYMORPHISM (RS1695) ILE 105 VAL FGB GENE IN PREGNANT WOMEN
Abstract
Introduction. Currently, the most implemented approach to the study of the mechanisms of the formation of obstetric complications (in particular, fetal growth restriction syndrome and fetoplacental insufficiency, FPI) is the identification of disease associations with DNA polymorphisms of candidate genes or their protein products. Particular attention is paid to the genes of the endothelial system, which play a role in the development of thrombophilia. Patients and methods. The study involved 50 pregnant women aged 20 to 45 years, including 40 patients with FPI and 10 patients without FPI. All pregnant women underwent general clinical, instrumental, biochemical and molecular genetic studies. The object and subject of research for molecular genetic studies were DNA samples of pregnant women and the FGB gene polymorphism (rs1695) IIe 105 Val. Results. The results of a comparative analysis of the frequencies of distribution of alleles and genotypes of IIe 105 Val polymorphism of the FGB fibrinolysis gene among 80 DNA samples in 40 pregnant women in 87.5% of cases revealed the presence of the normal allele G and in 12.5% of cases — the presence of the allele A (χ2=0.1; р=0.8; OR=1.2; 95%CI 0.306-4.983). Meanwhile, in the control group in 10 pregnant women without FPI, the frequency of the normal allele G of the FGB gene was 85%, while the frequency of the A mutant allele IIe 105 Val of the FGB gene amounted to 15%. The study of the genetic structure of this marker revealed a tendency to an increase in the expected mutation in the main group of pregnant women with FPI in relation to the group without FPI (10% and 2.25%, respectively). Conclusion. FGB fibrinolysis gene polymorphism (rs1695) IIe 105 Val is relatively widespread among pregnant women in Uzbekistan, and its clinical significance requires further studies.
