Морфологія (Jan 2009)
Research of some sides of antifungal activity mechanism act of new adamantane derivative
Abstract
Mechanism of the antiviral action of adamantane-containing preparations caused by their membranotropic properties. In the screening studies we discovered the substance 4-adamantyl-1(1-aminobutil) benzene (substance AM-166) with the wide spectrum of antimicrobic action. AM-166 suppresses increase and multiplication of bacteria (aerobic, anaerobic) and fungi (yeast like, mold and dermatomyces). The ointment for treating the pyoinflammatory processes was developed on the basis of adamantane-containing substance. The purpose of our experiments was to learn some sides of the mechanism of the antifungal action of substance AM-166 at the ultramicroscopic level. Experiments were carried out on the Candida albicans NCTC885/653. Substance concentration was 4 MIC. Ultrathin sections obtained on the ultratome LKB-880 U and contrasted by acetate of uranyl and by citrate of lead, were investigated in the electron microscope EM-125 K. It is established, that the substance AM-166 in concentration 4 MIC causes the damage of cell wall and cytoplasmic structures of the yeast-like fungus of C.albicans. These disturbances were recorded after 1 h of incubation with the substance AM-166. Thus, our experiments showed that the inhibiting action of adamantane-containing substance is caused, first of all, by influence on the cellular mem-brane of fungus.
