Nature Communications (Sep 2019)

CRISPR-Cas9-based mutagenesis frequently provokes on-target mRNA misregulation

  • Rubina Tuladhar,
  • Yunku Yeu,
  • John Tyler Piazza,
  • Zhen Tan,
  • Jean Rene Clemenceau,
  • Xiaofeng Wu,
  • Quinn Barrett,
  • Jeremiah Herbert,
  • David H. Mathews,
  • James Kim,
  • Tae Hyun Hwang,
  • Lawrence Lum

DOI
https://doi.org/10.1038/s41467-019-12028-5
Journal volume & issue
Vol. 10, no. 1
pp. 1 – 10

Abstract

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CRISPR-Cas9 genome editing is presumed to knock out gene function by generating a frameshift during NHEJ repair. Here, the authors investigate mRNA and protein expression in edited lines and find genome editing can generate internal ribosome entry sites or alternatively spliced variants.