Association Between Paraoxonase 1 (PON 1) And Apolipoprotein E (APO E) Polymorphism with Lipid Profile in Acute Coronary Syndrome Patients

Abstract

Objective: Acute coronary syndromes (ACS) are categorized by a sudden reduction in blood supply to the heart and include ST-segment elevation myocardial infarction (STEMI), non-STEMI (NSTEMI), and unstable angina. The aim of the present study is to evaluate whether the PON1 and APO E genes single nucleotide polymorphisms (SNPs) are associated with the presence of acute coronary syndrome (ACS) and lipid profiles in a case-control association study in an Iraqi Kurdistan population. Methods: We genotyped two single nucleotide polymorphisms (SNPs) in APOE and PON 1 genes and compared patients who had an initial ACS with patients who presented with stable exertional angina. Results: A total of 77 samples, including patients with ACS (N = 61), and those with chest pain syndrome (controls, N = 16) were enrolled. Genotyping for PON1 and APO E genes was performed using the PCR assay. A series of statistical analyses were performed to investigate the association between PON1 and APO E genes SNPs and the susceptibility to ACS. The results revealed a non-significant association of APOE with ACS risk in which £2 (P = 0.585) which was found in all patients and control except 2 patients and 1 control that carried £3 (P = 0.508). £3 was the risk factor, PON 1 was non significantly associated with ACS in which the A allele (P=0.781) in which mostly found in ACS patients in comparison to the control group and it’s a strong risk factor for the disease. Also, T allele (P=0.127) found mostly in ACS patients in comparison to the control group. Logistic regression analyses further revealed an increased risk for ACS in A allele carrier, when compared to those with TT homozygotes (odds ratio: 1.199, 95% CI 0.420-3.756, P = 0.781). Furthermore, there were not any significant differences between lipids profile with both genes (APO E and PON 1). The results of the present study suggested that the PON1 and APO E may play a role in the pathogenesis of Acute coronary syndrome in Kurdistan /Erbil population and that a decrease in PON1 activity may be a valuable marker for monitoring the development of the atherosclerosis process and the associated cardiovascular complications.

Keywords

• pon 1 gene
• apo e gene
• polymorphism
• lipid profiles
• acute coronary syndrome patients