In Vitro and In Vivo Photoprotective Effects of (-)-Loliode Isolated from the Brown Seaweed, <i>Sargassum horneri</i>
Lei Wang,
Hyun-Soo Kim,
Jun-Geon Je,
Xiaoting Fu,
Caoxing Huang,
Ginnae Ahn,
Jae-Young Oh,
K. K. Asanka Sanjeewa,
Jiachao Xu,
Xin Gao,
In-Kyu Yeo,
You-Jin Jeon
Affiliations
Lei Wang
College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China
Hyun-Soo Kim
National Marine Biodiversity Institute of Korea, 75, Jangsan-ro 101gil, Janghang-eup, Seocheon 33662, Korea
Jun-Geon Je
Department of Marine Life Sciences, Jeju National University, Jeju 63243, Korea
Xiaoting Fu
College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China
Caoxing Huang
Co-Innovation Center for Efficient Processing and Utilization of Forest Products, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China
Ginnae Ahn
Department of Marine Bio Food Science, Chonnam National University, Yeosu 59626, Korea
Jae-Young Oh
Food Safety and Processing Research Division, National Institute of Fisheries Science, Busan 46083, Korea
K. K. Asanka Sanjeewa
Department of Biosystem Technology, Faculty of Technology, University of Sri Jayewardenepura, Pitipana, Homagama 10206, Sri Lanka
Jiachao Xu
College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China
Xin Gao
College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China
In-Kyu Yeo
Department of Marine Life Sciences, Jeju National University, Jeju 63243, Korea
You-Jin Jeon
Department of Marine Life Sciences, Jeju National University, Jeju 63243, Korea
Skin is the largest organ of humans. Overexposure to ultraviolet (UV) is the primary environmental factor that causes skin damage. The compound, (-)-loliode, isolated from the brown seaweed Sargassum horneri, showed strong antioxidant and anti-inflammatory activities in in vitro and in vivo models. To further explore the potential of (-)-loliode in cosmetics, in the present study, we investigated the photoprotective effect of (-)-loliode in vitro in skin cells and in vivo in zebrafish. The results indicated that (-)-loliode significantly reduced intracellular reactive oxygen species (ROS) level, improved cell viability, and suppressed apoptosis of UVB-irradiated human keratinocytes. In addition, (-)-loliode remarkably attenuated oxidative damage, improved collagen synthesis, and inhibited matrix metalloproteinases expression in UVB-irradiated human dermal fibroblasts. Furthermore, the in vivo test demonstrated that (-)-loliode effectively and dose-dependently suppressed UVB-induced zebrafish damage displayed in decreasing the levels of ROS, nitric oxide, lipid peroxidation, and cell death in UVB-irradiated zebrafish. These results indicate that (-)-loliode possesses strong photoprotective activities and suggest (-)-loliode may an ideal ingredient in the pharmaceutical and cosmeceutical industries.
