Antibacterial Activity and Cytocompatibility of Electrospun PLGA Scaffolds Surface-Modified by Pulsed DC Magnetron Co-Sputtering of Copper and Titanium
Arsalan D. Badaraev,
Marat I. Lerner,
Olga V. Bakina,
Dmitrii V. Sidelev,
Tuan-Hoang Tran,
Maksim G. Krinitcyn,
Anna B. Malashicheva,
Elena G. Cherempey,
Galina B. Slepchenko,
Anna I. Kozelskaya,
Sven Rutkowski,
Sergei I. Tverdokhlebov
Affiliations
Arsalan D. Badaraev
Weinberg Research Center, School of Nuclear Science & Engineering, National Research Tomsk Polytechnic University, 30 Lenin Avenue, 634050 Tomsk, Russia
Marat I. Lerner
Institute of Strength Physics and Materials Sciences of Siberian Branch of the Russian Academy of Sciences, 2/4 Akademicheskii Avenue, 634055 Tomsk, Russia
Olga V. Bakina
Institute of Strength Physics and Materials Sciences of Siberian Branch of the Russian Academy of Sciences, 2/4 Akademicheskii Avenue, 634055 Tomsk, Russia
Dmitrii V. Sidelev
Weinberg Research Center, School of Nuclear Science & Engineering, National Research Tomsk Polytechnic University, 30 Lenin Avenue, 634050 Tomsk, Russia
Tuan-Hoang Tran
Weinberg Research Center, School of Nuclear Science & Engineering, National Research Tomsk Polytechnic University, 30 Lenin Avenue, 634050 Tomsk, Russia
Maksim G. Krinitcyn
Weinberg Research Center, School of Nuclear Science & Engineering, National Research Tomsk Polytechnic University, 30 Lenin Avenue, 634050 Tomsk, Russia
Anna B. Malashicheva
Institute of Cytology RAS, 4 Tikhoretsky Avenue, 194064 Saint Petersburg, Russia
Elena G. Cherempey
Weinberg Research Center, School of Nuclear Science & Engineering, National Research Tomsk Polytechnic University, 30 Lenin Avenue, 634050 Tomsk, Russia
Galina B. Slepchenko
Weinberg Research Center, School of Nuclear Science & Engineering, National Research Tomsk Polytechnic University, 30 Lenin Avenue, 634050 Tomsk, Russia
Anna I. Kozelskaya
Weinberg Research Center, School of Nuclear Science & Engineering, National Research Tomsk Polytechnic University, 30 Lenin Avenue, 634050 Tomsk, Russia
Sven Rutkowski
Weinberg Research Center, School of Nuclear Science & Engineering, National Research Tomsk Polytechnic University, 30 Lenin Avenue, 634050 Tomsk, Russia
Sergei I. Tverdokhlebov
Weinberg Research Center, School of Nuclear Science & Engineering, National Research Tomsk Polytechnic University, 30 Lenin Avenue, 634050 Tomsk, Russia
Biocompatible poly(lactide-co-glycolide) scaffolds fabricated via electrospinning are having promising properties as implants for the regeneration of fast-growing tissues, which are able to degrade in the body. The hereby-presented research work investigates the surface modification of these scaffolds in order to improve antibacterial properties of this type of scaffolds, as it can increase their application possibilities in medicine. Therefore, the scaffolds were surface-modified by means of pulsed direct current magnetron co-sputtering of copper and titanium targets in an inert atmosphere of argon. In order to obtain different amounts of copper and titanium in the resulting coatings, three different surface-modified scaffold samples were produced by changing the magnetron sputtering process parameters. The success of the antibacterial properties’ improvement was tested with the methicillin-resistant bacterium Staphylococcus aureus. In addition, the resulting cell toxicity of the surface modification by copper and titanium was examined using mouse embryonic and human gingival fibroblasts. As a result, the scaffold samples surface-modified with the highest copper to titanium ratio show the best antibacterial properties and no toxicity against mouse fibroblasts, but have a toxic effect to human gingival fibroblasts. The scaffold samples with the lowest copper to titanium ratio display no antibacterial effect and toxicity. The optimal poly(lactide-co-glycolide) scaffold sample is surface-modified with a medium ratio of copper and titanium that has antibacterial properties and is non-toxic to both cell cultures.