Mito-SiPE is a sequence-independent and PCR-free mtDNA enrichment method for accurate ultra-deep mitochondrial sequencing

Darren J. Walsh; David J. Bernard; Faith Pangilinan; Madison Esposito; Denise Harold; Anne Parle-McDermott; Lawrence C. Brody

doi:10.1038/s42003-022-04182-2

Communications Biology (Nov 2022)

Mito-SiPE is a sequence-independent and PCR-free mtDNA enrichment method for accurate ultra-deep mitochondrial sequencing

Darren J. Walsh,
David J. Bernard,
Faith Pangilinan,
Madison Esposito,
Denise Harold,
Anne Parle-McDermott,
Lawrence C. Brody

Affiliations

Darren J. Walsh: Gene and Environment Interaction Section, National Human Genome Research Institute, NIH
David J. Bernard: Gene and Environment Interaction Section, National Human Genome Research Institute, NIH
Faith Pangilinan: Gene and Environment Interaction Section, National Human Genome Research Institute, NIH
Madison Esposito: Gene and Environment Interaction Section, National Human Genome Research Institute, NIH
Denise Harold: School of Biotechnology, Dublin City University
Anne Parle-McDermott: School of Biotechnology, Dublin City University
Lawrence C. Brody: Gene and Environment Interaction Section, National Human Genome Research Institute, NIH

DOI: https://doi.org/10.1038/s42003-022-04182-2
Journal volume & issue: Vol. 5, no. 1
pp. 1 – 11

Abstract

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Mito-SiPE is a mitochondrial DNA (mtDNA) enrichment method that can derive high-quality mtDNA for rare heteroplasmy analysis, without relying on PCR or probes.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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