Association between angiotensinogen M235T gene polymorphism and risk of ischemic stroke among the Ethiopian population: a case control study

Abstract

Ischemic stroke (IS) is a multifaceted, complicated illness resulting from a confluence of genetic, environmental and vascular risk factors. Many genes that may contribute to ischemic stroke have been discovered in humans. The genetic contribution appears to be greater in IS patients with hypertension. There is conflicting evidence about a positive correlation between the AGT M235T polymorphism and ischemic stroke. The aim of this study was to examine the possible association of the AGT M235T gene polymorphism with the risk of ischemic stroke. A hospital-based case-control study was carried out in 36 ischemic stroke patient cases and 36 age- and sex-matched healthy controls. Clinical parameters were measured to assess the associated risk factors. DNA was isolated from blood samples, and the AGT M235T genotypes were identified using polymerase chain reaction (PCR) and analyzed by agarose gel electrophoresis. The AGT-TT genotype (OR = 4.64, 95% CL = 1.23–17.4; p = 0.023 and T allele (OR = 2.54, 95% CL = 1.28–5.02; p = 0.003) were significantly more common in patients than in controls, indicating that it may be a major risk factor for the development of ischemic stroke. The results suggest that there may be a significant correlation between the AGT M235T gene polymorphism and the development of ischemic stroke in the studied Ethiopian population.

Keywords

• Angiotensinogen
• clinical risk factors
• genotype
• ischemic stroke