Transfection of clMagR/clCry4 imparts MR-T2 imaging contrast properties to living organisms (E. coli) in the presence of Fe3+ by endogenous formation of iron oxide nanoparticles

Nuan Li; Nuan Li; Le Xue; Le Xue; Xiaoli Mai; Peng Wang; Peng Wang; Peng Wang; Chenzhuo Zhu; Xiaofeng Han; Yuanyuan Xie; Bin Wang; Yuqing Ge; Yewei Zhang; Jianfei Sun; Jianfei Sun

doi:10.3389/fmolb.2023.1119356

Frontiers in Molecular Biosciences (Feb 2023)

Transfection of clMagR/clCry4 imparts MR-T2 imaging contrast properties to living organisms (E. coli) in the presence of Fe3+ by endogenous formation of iron oxide nanoparticles

Nuan Li,
Nuan Li,
Le Xue,
Le Xue,
Xiaoli Mai,
Peng Wang,
Peng Wang,
Peng Wang,
Chenzhuo Zhu,
Xiaofeng Han,
Yuanyuan Xie,
Bin Wang,
Yuqing Ge,
Yewei Zhang,
Jianfei Sun,
Jianfei Sun

Affiliations

Nuan Li: State Key Laboratory of Bioelectronics, School of Biological Sciences and Medical Engineering, Southeast University, Nanjing, China
Nuan Li: Jiangsu Key Laboratory of Biomaterials and Devices, Southeast University, Nanjing, China
Le Xue: State Key Laboratory of Bioelectronics, School of Biological Sciences and Medical Engineering, Southeast University, Nanjing, China
Le Xue: Jiangsu Key Laboratory of Biomaterials and Devices, Southeast University, Nanjing, China
Xiaoli Mai: Department of Radiology, The Affiliated Drum Tower Hospital of Nanjing University, Nanjing, China
Peng Wang: State Key Laboratory of Bioelectronics, School of Biological Sciences and Medical Engineering, Southeast University, Nanjing, China
Peng Wang: Jiangsu Key Laboratory of Biomaterials and Devices, Southeast University, Nanjing, China
Peng Wang: Department of Sports Medicine and Adult Reconstructive Surgery, The Affiliated Drum Tower Hospital of Nanjing University, Nanjing, China
Chenzhuo Zhu: Southeast University-Monash University Joint Graduate School, Southeast University, Suzhou, China
Xiaofeng Han: State Key Laboratory of Bioelectronics, School of Biological Sciences and Medical Engineering, Southeast University, Nanjing, China
Yuanyuan Xie: Clinical Stem Cell Center, The Affiliated Drum Tower Hospital of Nanjing University, Nanjing, China
Bin Wang: Clinical Stem Cell Center, The Affiliated Drum Tower Hospital of Nanjing University, Nanjing, China
Yuqing Ge: State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai, China
Yewei Zhang: The Hepatopancreatobiliary Center, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, China
Jianfei Sun: State Key Laboratory of Bioelectronics, School of Biological Sciences and Medical Engineering, Southeast University, Nanjing, China
Jianfei Sun: Jiangsu Key Laboratory of Biomaterials and Devices, Southeast University, Nanjing, China

DOI: https://doi.org/10.3389/fmolb.2023.1119356
Journal volume & issue: Vol. 10

Abstract

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Rapid development of medical imaging, such as cellular tracking, has increased the demand for “live” contrast agents. This study provides the first experimental evidence demonstrating that transfection of the clMagR/clCry4 gene can impart magnetic resonance imaging (MRI) T2-contrast properties to living prokaryotic Escherichia coli (E. coli) in the presence of Fe3+ through the endogenous formation of iron oxide nanoparticles. The transfected clMagR/clCry4 gene markedly promoted uptake of exogenous iron by E. coli, achieving an intracellular co-precipitation condition and formation of iron oxide nanoparticles. This study will stimulate further exploration of the biological applications of clMagR/clCry4 in imaging studies.

Published in Frontiers in Molecular Biosciences

ISSN: 2296-889X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Biology (General)
Website: https://www.frontiersin.org/journals/molecular-biosciences

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