Frontiers in Bioengineering and Biotechnology (Jan 2023)

Synergistic remineralization of enamel white spot lesions using mesoporous bioactive glasses loaded with amorphous calcium phosphate

  • Juan Ren,
  • Juan Ren,
  • Jianping Rao,
  • Jianping Rao,
  • He Wang,
  • He Wang,
  • Wenjing He,
  • Jinnan Feng,
  • Jinnan Feng,
  • Danni Wei,
  • Danni Wei,
  • Bin Zhao,
  • Bin Zhao,
  • Xing Wang,
  • Xing Wang,
  • Wei Bian

DOI
https://doi.org/10.3389/fbioe.2023.1109195
Journal volume & issue
Vol. 11

Abstract

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Objectives: The purpose of this study was to create a new delivery system that can synergistically remineralize enamel white spot lesions (WSLs).Materials and methods: The delivery system (PAA-ACP@aMBG) was prepared by using aminated mesoporous bioactive glasses (aMBG) as the carrier loaded with polyacrylic-stabilized amorphous calcium phosphate (PAA-ACP). The materials were characterized by transmission electron microscopy (TEM), X-ray powder diffraction (XRD), inductively coupled plasma–optical emission spectrometry (ICP–OES), and so on. Forty-eight artificial WSLs enamel samples were randomized to four groups: artificial saliva (negative control, NC), casein phosphopeptide-amorphous calcium phosphate (CPP-ACP), PAA-ACP@aMBG, and MBG. The effects of demineralization and remineralization of the enamel surface were compared by means of surface microhardness (SMH) measurements, surface color change measurements, fluorescence microscopy (FM), X-ray diffraction (XRD) analysis and scanning electron microscopy (SEM).Results: There was no significant difference in the surface microhardness recovery rate (SMHRR) or color recovery rate (CRR) among the CPP-ACP group, PAA-ACP@aMBG group and MBG group (P>0.05), but these values were significantly higher than those in the NC group (p < 0.01). FM demonstrated that the remineralization depth in the PAA-ACP@aMBG group was significantly greater than that of the remaining three groups (p < 0.01). SEM analysis indicated that the enamel demineralization marks in the PAA-ACP@aMBG group, CPP-ACP group, and MBG group were obscured by mineral deposition.Conclusions: PAA-ACP@aMBG showed good mineralization properties, implying its great potential for clinical application.

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