Detection of sparfloxacin based on water-soluble CuInS2 quantum dots

Abstract

Thioglycolic acid-modified CuInS2 quantum dots were prepared by a hydrothermal method. A method for the determination of sparfloxacin by a CuInS2 ternary-quantum dot fluorescence probe was established based on the fact that the fluorescence of CuInS2 quantum dots can be significantly quenched by sparfloxacin. The optimized experimental conditions are as follows: (1) the concentration of CuInS2 quantum dots is 0.4 mol·L−1; (2) the pH of the buffer solution is 6, and 1 mL of buffer was used; (3) the reaction time is 5 min. The concentration of sparfloxacin is linearly related to the fluorescence quenching intensity from 0.5 μg/mL to 1 mg/mL, and the maximum detection limit is 1 mg/mL.

Keywords

• Sparfloxacin
• Quantum dots
• Fluorescence