Serum Raman spectroscopy: Unearthing the snapshot of distinct metabolic profile in patients with congenital heart defects (CHDs)
Radha Joshi,
Debosmita Goswami,
Panchali Saha,
Arti Hole,
Poonam Mandhare,
Rishikesh Wadke,
Prabhatha Rashmi Murthy,
Shyamdeep Borgohain,
Murali Krishna C,
Sudhir Kapoor
Affiliations
Radha Joshi
Sri Sathya Sai Sanjeevani Research Centre, Sri Sathya Sai Sanjeevani Research Foundation, Plot No. 2, Sector 38, Kharghar, Navi Mumbai, 410210, Maharashtra, India
Debosmita Goswami
Advanced Centre for Treatment, Research and Education in Cancer (ACTREC), Sector 22, Utsav Chowk - CISF Road, Owe Camp, Kharghar, Navi Mumbai, 410210, Maharashtra, India
Panchali Saha
Advanced Centre for Treatment, Research and Education in Cancer (ACTREC), Sector 22, Utsav Chowk - CISF Road, Owe Camp, Kharghar, Navi Mumbai, 410210, Maharashtra, India; Training School Complex, Homi Bhabha National Institute, Anushakti Nagar, Maharashtra, India
Arti Hole
Advanced Centre for Treatment, Research and Education in Cancer (ACTREC), Sector 22, Utsav Chowk - CISF Road, Owe Camp, Kharghar, Navi Mumbai, 410210, Maharashtra, India
Poonam Mandhare
Sri Sathya Sai Sanjeevani Research Centre, Sri Sathya Sai Sanjeevani Research Foundation, Plot No. 2, Sector 38, Kharghar, Navi Mumbai, 410210, Maharashtra, India
Rishikesh Wadke
Sri Sathya Sai Sanjeevani Centre for Child Heart Care & Training in Pediatric Cardiac Skills, Plot No. 2, Sector 38, Kharghar, Navi Mumbai, 410210, Maharashtra, India
Prabhatha Rashmi Murthy
Sri Sathya Sai Sanjeevani Centre for Child Heart Care & Training in Pediatric Cardiac Skills, Plot No. 2, Sector 38, Kharghar, Navi Mumbai, 410210, Maharashtra, India
Shyamdeep Borgohain
Sri Sathya Sai Sanjeevani Centre for Child Heart Care & Training in Pediatric Cardiac Skills, Plot No. 2, Sector 38, Kharghar, Navi Mumbai, 410210, Maharashtra, India
Murali Krishna C
Advanced Centre for Treatment, Research and Education in Cancer (ACTREC), Sector 22, Utsav Chowk - CISF Road, Owe Camp, Kharghar, Navi Mumbai, 410210, Maharashtra, India; Training School Complex, Homi Bhabha National Institute, Anushakti Nagar, Maharashtra, India; Corresponding author. Advanced Centre for Treatment Research and Education in Cancer, Tata Memorial Centre (TMC), Kharghar, Navi Mumbai, 410210, India.
Sudhir Kapoor
Sri Sathya Sai Sanjeevani Research Centre, Sri Sathya Sai Sanjeevani Research Foundation, Plot No. 2, Sector 38, Kharghar, Navi Mumbai, 410210, Maharashtra, India; Corresponding author. Sri Sathya Sai Sanjeevani Research Foundation, Sector 38, Kharghar, Navi Mumbai, 410210, India.
In the present study, efficacy of minimally-invasive serum Raman spectroscopy (SRS) in stratification of congenital heart diseases was explored. Blood was collected from 62 subjects [42 congenital heart defect (CHD) patients (19 with atrial septal defect, 13 with ventricular septal defect and 10 with tetralogy of fallot) and 20 controls], and serum separated. Raman spectra of sera were recorded, pre-processed and subjected to spectral and multivariate analyses. Multivariate curve resolution-alternating least squares (MCR-ALS) analyses indicated alterations in lipid and protein levels between the study groups. Principal Component Analysis (PCA) and Principal Component based Linear Discriminant Analysis (PC-LDA), cross-validated with Leave-one-out cross validation (LOOCV), were employed to study stratification between the different groups. CHD could be classified from controls with 76 % efficiency. The different CHD subtypes could be distinguished with efficiencies as high as ∼90 %. To the best of our knowledge, differentiation between controls and CHDs as well as the stratification between controls and CHDs subtypes was for the first time successfully accomplished by serum-based Raman spectroscopy.
