Fungal endophyte diversity in the leaves of the medicinal plant Myracrodruon urundeuva in a Brazilian dry tropical forest and their capacity to produce L-asparaginase

Acta Botânica Brasílica. 2018;(0) DOI 10.1590/0102-33062018abb0108


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Journal Title: Acta Botânica Brasílica

ISSN: 0102-3306 (Print); 1677-941X (Online)

Publisher: Sociedade Botânica do Brasil

Society/Institution: Sociedade Botânica do Brasil, SBB

LCC Subject Category: Science: Botany

Country of publisher: Brazil

Language of fulltext: English

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Ana Patrícia Sousa Lopes de Pádua

Karla Torres Lins de Sousa Freire

Thays Gabrielle Lins de Oliveira

Leticia Francisca da Silva

Gianne Rizzuto Araújo-Magalhães

Gualberto Segundo Agamez-Montalvo

Iolanda Ramalho da Silva

Jadson Diogo Pereira Bezerra

Cristina Maria de Souza-Motta


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Time From Submission to Publication: 18 weeks


Abstract | Full Text

ABSTRACT We studied the fungal endophyte community of the leaves of Myracrodruon urundeuva in Brazil along with their potential to produce L-asparaginase. In total, 187 endophytes were isolated. The colonization rate of leaf fragments from caatinga (10.41 %) was lower than those from brejo de altitude (39.58 %). Sequences of ITS rDNA from all endophytes revealed relationships with Ascomycota (Botryosphaeriales, Chaetothyriales, Diaporthales, Eurotiales, Glomerellales, Hypocreales, and Pleosporales) and Basidiomycota (Polyporales). The most frequent endophytes were members of the genus Diaporthe. Talaromyces was an indicator genus for caatinga while Phyllosticta, Diaporthe, and Colletotrichum were for brejo de altitude. The composition of endophytic fungi in caatinga and brejo de altitude differed significantly, its richness and diversity (H' = 3.41) being significantly higher in brejo de altitude. Diaporthe sp. URM 7793 was the best producer of L-asparaginase (2.41 U/g), followed by Diaporthe sp. URM 7779 (2.00 U/g), Talaromyces sp. URM 7785 (1.91 U/g), and Diaporthe sp. URM 7792 (1.47 U/g). The composition of endophytic fungi assemblages is strongly influenced by the type of ecosystem. In its natural habitat, M. urundeuva harbors an important diversity of endophytes, which could be used to produce L-asparaginase.