Journal of Pharmacological Sciences (Mar 2022)

Noradrenaline increases intracellular Ca2+ concentration in epithelial cells via α2-adrenoceptors in isolated mouse ileal crypts

  • Tomoko Kimyo,
  • Takuji Machida,
  • Kenji Iizuka,
  • Masaru Minami,
  • Masahiko Hirafuji

DOI
https://doi.org/10.1016/j.jphs.2022.01.010
Journal volume & issue
Vol. 148, no. 3
pp. 337 – 341

Abstract

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The stimulation of α2-adrenoceptors caused a transient increase of intracellular calcium concentration ([Ca2+]i) monitored by ratiometry using Fura-2 in epithelial cells including enterochromaffin cells in isolated mouse ileal crypts, while stimulation of α1-and β-adrenoceptors had no effect. The effect of noradrenaline was suppressed by α2-adrenoceptor antagonists, but not by α1-and β-adrenoceptor antagonists, and partially suppressed by Ni2+ and nicardipine, but not by ω-conotoxin and ω-agatoxin. These results suggest that noradrenaline causes an increase of [Ca2+]i by the influx of extracellular Ca2+ through certain Ca2+ channels via α2-adrenoceptors in epithelial cells of mouse ileal crypts.

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