An Optimized Terpene Profile for a New Medical Cannabis Oil
Valentina Maggini,
Lorenzo Calvi,
Tommaso Pelagatti,
Eugenia Rosaria Gallo,
Celine Civati,
Carlo Privitera,
Flavio Squillante,
Paolo Maniglia,
Domenico Di Candia,
Roberto Spampatti,
Fabio Firenzuoli
Affiliations
Valentina Maggini
Research and Innovation Center in Phytotherapy and Integrated Medicine (CERFIT), Careggi University Hospital, Via Delle Oblate 4, 50141 Florence, Italy
Lorenzo Calvi
Research and Innovation Center in Phytotherapy and Integrated Medicine (CERFIT), Careggi University Hospital, Via Delle Oblate 4, 50141 Florence, Italy
Tommaso Pelagatti
Research and Innovation Center in Phytotherapy and Integrated Medicine (CERFIT), Careggi University Hospital, Via Delle Oblate 4, 50141 Florence, Italy
Eugenia Rosaria Gallo
Research and Innovation Center in Phytotherapy and Integrated Medicine (CERFIT), Careggi University Hospital, Via Delle Oblate 4, 50141 Florence, Italy
Celine Civati
Farmacia Tili, Piazza Vittorio Veneto 32, 22036 Erba, Italy
Carlo Privitera
Progetto MediCOmm s.r.l.s., C.da due Fontane s.n., 93100 Caltanissetta, Italy
Flavio Squillante
Farmacia Tili, Piazza Vittorio Veneto 32, 22036 Erba, Italy
Paolo Maniglia
Department of Anesthesia and Intensive Care Medicine, Alessandro Manzoni Hospital, Azienda Socio-Sanitaria Territoriale Lecco, 23900 Lecco, Italy
Domenico Di Candia
Department of Biomedical Sciences for Health, Section of Legal Medicine, University of Milan, 20133 Milan, Italy
Roberto Spampatti
Farmacia Tili, Piazza Vittorio Veneto 32, 22036 Erba, Italy
Fabio Firenzuoli
Research and Innovation Center in Phytotherapy and Integrated Medicine (CERFIT), Careggi University Hospital, Via Delle Oblate 4, 50141 Florence, Italy
The purpose of this analytical study was to develop an advanced formulation of medical Cannabis oil (MCO) comparing the chemical profile of different extracts obtained with two existing methods (SIFAP and CALVI) and one original upgraded (CERFIT) method. Preparation methods were applied with varying solvent, temperature, and duration of the decarboxylation and extraction steps. HPLC-MS/MS TSQ and GC/FID-HS analyses were performed to investigate cannabinoid and terpene contents in the three oil extracts. Cannabinoids profile remained comparable between the formulations. CERFIT extracts exhibited a superior quantity of total terpene hydrocarbon forms (e.g., limonene and α-pinene) with no degradation occurrence (i.e., oxidized terpenes not quantifiable). Thus, this new method optimized the phytochemical profile of the MCO presenting a value opportunity to obtain a standardized high-level therapeutic product.
