Hydraena (s.str.) dinarica, new species (Coleoptera: Hydraenidae) along with further records of Hydraena spp. from Durmitor National Park, Montenegro and comments on the DNA barcoding problem with the genus

Abstract

Long-palped Water Beetles were collected during a taxon expedition in Montenegro which involved citizen scientists, students and taxonomists. The material was collected from springs, brooks, fens and the Tara River, at altitudes between 600m and 1450 m above sea level, using fine-meshed hand-nets and by manual checking of submerged substrates. The morphological species delimitation was supplemented and congruent with mtDNA sequences mainly obtained in the field using the newly developed MinION-based ONTrack pipeline.The new species Hydraena dinarica Freitag & de Vries, sp. nov. from the Durmitor Mt. is described, illustrated and compared in detail to closely related congeners of the H. saga (d'Orchymont, 1930) / H. emarginata Rey, 1885 species complex. Five additional species and female specimens of two unidentified morphospecies of the genus were also recorded in the vicinity of Durmitor National Park. New records and the first DNA barcodes for Hydraena biltoni Jäch & Díaz, 2012 (endemic to Montenegro) and H. morio Kiesenwetter, 1849 are provided. Further records of H. nigrita Germar, 1824; H. minutissima Stephens, 1829; H. subintegra Ganglbauer, 1901 and females of two unidentified morphospecies are commented. The the resulting inter- and intraspecific genetic distances, and some observations of low or zero sequence divergence between recently diverged species of Hydraena Kugelann, 1794 are briefly discussed.

Keywords

• citizen science
• MinION sequencing
• minute moss be