PLoS ONE (Jan 2011)

Using time-resolved fluorescence to measure serum venom-specific IgE and IgG.

  • Pauline E van Eeden,
  • Michael D Wiese,
  • Susan Aulfrey,
  • Belinda J Hales,
  • Shelley F Stone,
  • Simon G A Brown

DOI
https://doi.org/10.1371/journal.pone.0016741
Journal volume & issue
Vol. 6, no. 1
p. e16741

Abstract

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We adapted DELFIA™ (dissociation-enhanced lanthanide fluoroimmunoassay), a time resolved fluorescence method, to quantitate whole venom specific and allergenic peptide-specific IgE (sIgE), sIgG(1) and sIgG(4) in serum from people clinically allergic to Australian native ant venoms, of which the predominant cause of allergy is jack jumper ant venom (JJAV). Intra-assay CV was 6.3% and inter-assay CV was 13.7% for JJAV sIgE. DELFIA and Phadia CAP JJAV sIgE results correlated well and had similar sensitivity and specificity for the detection of JJAV sIgE against intradermal skin testing as the gold standard. DELFIA was easily adapted for detecting sIgE to a panel of other native ant venoms.