Journal of Asthma and Allergy (Sep 2022)
IL-13 Regulates Orai1 Expression in Human Bronchial Smooth Muscle Cells and Airway Remodeling in Asthma Mice Model via LncRNA H19
Abstract
Lin-Li Xiang,1,* Qian-Qian Wan,2,* Yi-Min Wang,1 Shao-Jun He,3 Wen-Juan Xu,3 Mei Ding,1,4 Jin-Jin Zhang,1,4 Yuan-Li Sun,1,4 Xiang Dong,1,4 Ying Zhou,5 Yu-Bao Cui,6 Ya-Dong Gao1,4 1Department of Allergology, Zhongnan Hospital of Wuhan University, Wuhan, People’s Republic of China; 2Department of Rheumatology, Zhongnan Hospital of Wuhan University, Wuhan, People’s Republic of China; 3Department of Respiratory and Critical Care Medicine Zhongnan Hospital of Wuhan University, Wuhan, People’s Republic of China; 4Hubei Province Key Laboratory of Allergy and Immunology, Wuhan University, Wuhan, People’s Republic of China; 5Department of Pediatrics Laboratory, The Affiliated Wuxi Children’s Hospital of Nanjing Medical University, Wuxi, People’s Republic of China; 6Department of Clinical Laboratory, The Affiliated Wuxi People’s Hospital of Nanjing Medical University, Wuxi, People’s Republic of China*These authors contributed equally to this workCorrespondence: Ya-Dong Gao, Department of Allergology, Zhongnan Hospital of Wuhan University, Donoghue Road 169, Wuhan, People’s Republic of China, Email [email protected]: Increased proliferation and hypertrophy of airway smooth muscle cells (ASMCs) contribute substantially to airway remodeling in asthma. Interleukin (IL)-13 regulates ASMC proliferation by increasing Orai1 expression, the pore-forming subunit of store-operated Ca2+ entry (SOCE). The underlying mechanisms of this effect are not fully understood.Methods: Bioinformatic analysis identified an interaction between microRNA 93– 5p (miR-93-5p) and long non-coding RNA (lncRNA) H19, and between miR-93-5p and Orai1. RNA interference was used to investigate H19 knockdown on IL-13-induced proliferation and migration of in vitro cultured human bronchial smooth muscle cells (hBSMCs). Functional relevance of H19 in airway inflammation and airway remodeling was investigated in murine models of acute and chronic asthma.Results: IL-13 concentration-dependently increased the expression of H19 and Orai1 and decreased the expression of miR-93-5p in hBSMCs. H19 knockdown partly reversed the effects of IL-13 on the expression of miR-93-5p and Orai1 and attenuated the proliferation and migration of hBSMCs promoted by IL-13. IL-13-promoted expression of Orai1 was attenuated by miR-93-5p mimic and increased by miR-93-5p inhibitor. IL-13-promoted proliferation of hBSMCs was increased by miR-93-5p inhibitor but not affected by miR-93-5p mimic, whereas IL-13-promoted migration of hBSMCs was increased by miR-93-5p inhibitor and attenuated by miR-93-5p mimic. The inhibiting effect of H19 knockdown on IL-13-induced Orai1 expression and the proliferation and migration of hBSMCs was counteracted by miR-93-5p inhibitor but only marginally or not impacted by miR-93-5p mimic. The expression of H19 and Orai1 was higher in the lungs of asthmatic mice than in control mice. In asthmatic mice, H19 siRNA reduced Orai1 expression, inflammatory cell infiltration, goblet cell hyperplasia, collagen deposition and smooth muscle mass in the lungs.Conclusion: H19 may mediate the effects of IL-13 on Orai1 expression by inhibition of miR-93-5p in hBSMCs. H19 may be a therapeutic target for airway inflammation and airway remodeling.Keywords: IL-13, Orai1, lncRNA H19, microRNA-93-5p, bronchial smooth muscle cells, asthma model
