The impact of glutamine deprivation on the expression of MEIS3, SPAG4, LHX1, LHX2, and LHX6 genes in ERN1 knockdown U87 glioma cells

Krasnytska Dariia A.; Khita Olena O.; Tsymbal Dariia O.; Luzina Olha Y.; Cherednychenko Anastasiia A.; Kozynkevich Halyna E.; Bezrodny Borys H.; Minchenko Dmytro O.

doi:10.2478/enr-2022-0005

Endocrine Regulations (Jan 2022)

The impact of glutamine deprivation on the expression of MEIS3, SPAG4, LHX1, LHX2, and LHX6 genes in ERN1 knockdown U87 glioma cells

Krasnytska Dariia A.,
Khita Olena O.,
Tsymbal Dariia O.,
Luzina Olha Y.,
Cherednychenko Anastasiia A.,
Kozynkevich Halyna E.,
Bezrodny Borys H.,
Minchenko Dmytro O.

Affiliations

Krasnytska Dariia A.: Department of Molecular Biology, Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv, Ukraine
Khita Olena O.: Department of Molecular Biology, Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv, Ukraine
Tsymbal Dariia O.: Department of Molecular Biology, Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv, Ukraine
Luzina Olha Y.: Department of Molecular Biology, Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv, Ukraine
Cherednychenko Anastasiia A.: Department of Molecular Biology, Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv, Ukraine
Kozynkevich Halyna E.: Department of Pediatrics and Department of Surgery, National Bohomolets Medical University, Kyiv, Ukraine
Bezrodny Borys H.: Department of Pediatrics and Department of Surgery, National Bohomolets Medical University, Kyiv, Ukraine
Minchenko Dmytro O.: Department of Molecular Biology, Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv, Ukraine

DOI: https://doi.org/10.2478/enr-2022-0005
Journal volume & issue: Vol. 56, no. 1
pp. 38 – 47

Abstract

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Objective. The aim of the current study was to investigate the expression of genes encoded homeobox proteins such as MEIS3 (Meis homeobox 3), SPAG4 (sperm associated antigen 4), LHX1 (LIM homeobox 1), LHX2, and LHX6 in U87 glioma cells in response to glutamine deprivation in control glioma cells and cells with knockdown of ERN1 (endoplasmic reticulum to nucleus signaling 1), the major pathway of the endoplasmic reticulum stress signaling, for evaluation of a possible dependence on the expression of these important regulatory genes from glutamine supply and ERN1 signaling.

Published in Endocrine Regulations

ISSN: 1336-0329 (Online)
Publisher: Sciendo
Country of publisher: Poland
LCC subjects: Medicine: Internal medicine: Specialties of internal medicine: Diseases of the endocrine glands. Clinical endocrinology
Website: https://sciendo.com/journal/ENR

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