A time-resolved fluorescence microsphere-lateral flow immunochromatographic strip for quantitative detection of Pregnanediol-3-glucuronide in urine samples

Jiasheng Lin; Jiasheng Lin; Sanhua Li; Benchen Ye; Benchen Ye; Weigang Zheng; Weigang Zheng; Huihui Wang; Huihui Wang; Ying Liu; Ying Liu; Dong Wang; Dong Wang; Zaihui Wu; Zaihui Wu; Wen-Fei Dong; Wen-Fei Dong; Minghui Zan; Minghui Zan; Minghui Zan

doi:10.3389/fbioe.2023.1308725

Frontiers in Bioengineering and Biotechnology (Dec 2023)

A time-resolved fluorescence microsphere-lateral flow immunochromatographic strip for quantitative detection of Pregnanediol-3-glucuronide in urine samples

Jiasheng Lin,
Jiasheng Lin,
Sanhua Li,
Benchen Ye,
Benchen Ye,
Weigang Zheng,
Weigang Zheng,
Huihui Wang,
Huihui Wang,
Ying Liu,
Ying Liu,
Dong Wang,
Dong Wang,
Zaihui Wu,
Zaihui Wu,
Wen-Fei Dong,
Wen-Fei Dong,
Minghui Zan,
Minghui Zan,
Minghui Zan

Affiliations

Jiasheng Lin: CAS Key Laboratory of Biomedical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Science (CAS), Suzhou, China
Jiasheng Lin: Zhengzhou Institute of Biomedical Engineering and Technology, Zhengzhou, China
Sanhua Li: Henan Province Joint International Laboratory for Bioconjugation and Antibody Coupling, Zhengzhou, China
Benchen Ye: Zhengzhou Institute of Biomedical Engineering and Technology, Zhengzhou, China
Benchen Ye: Zhongke Technology Achievement Transfer and Transformation Center of Henan Province, Zhengzhou, China
Weigang Zheng: Zhengzhou Institute of Biomedical Engineering and Technology, Zhengzhou, China
Weigang Zheng: Zhongke Technology Achievement Transfer and Transformation Center of Henan Province, Zhengzhou, China
Huihui Wang: Zhengzhou Institute of Biomedical Engineering and Technology, Zhengzhou, China
Huihui Wang: Zhongke Technology Achievement Transfer and Transformation Center of Henan Province, Zhengzhou, China
Ying Liu: CAS Key Laboratory of Biomedical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Science (CAS), Suzhou, China
Ying Liu: Zhengzhou Institute of Biomedical Engineering and Technology, Zhengzhou, China
Dong Wang: CAS Key Laboratory of Biomedical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Science (CAS), Suzhou, China
Dong Wang: Zhengzhou Institute of Biomedical Engineering and Technology, Zhengzhou, China
Zaihui Wu: CAS Key Laboratory of Biomedical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Science (CAS), Suzhou, China
Zaihui Wu: Zhengzhou Institute of Biomedical Engineering and Technology, Zhengzhou, China
Wen-Fei Dong: CAS Key Laboratory of Biomedical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Science (CAS), Suzhou, China
Wen-Fei Dong: Zhengzhou Institute of Biomedical Engineering and Technology, Zhengzhou, China
Minghui Zan: CAS Key Laboratory of Biomedical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Science (CAS), Suzhou, China
Minghui Zan: Zhengzhou Institute of Biomedical Engineering and Technology, Zhengzhou, China
Minghui Zan: Zhongke Technology Achievement Transfer and Transformation Center of Henan Province, Zhengzhou, China

DOI: https://doi.org/10.3389/fbioe.2023.1308725
Journal volume & issue: Vol. 11

Abstract

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Introduction: Pregnanediol-3-glucuronide (PdG), as the main metabolite of progesterone in urine, plays a significant role in the prediction of ovulation, threatened abortion, and menstrual cycle maintenance.Methods: To achieve a rapid and sensitive assay, we have designed a competitive model-based time-resolved fluorescence microsphere-lateral flow immunochromatography (TRFM-LFIA) strip.Results: The optimized TRFM-LFIA strip exhibited a wonderful response to PdG over the range of 30–2,000 ng/mL, the corresponding limit of detection (LOD) was calculated as low as 8.39 ng/mL. More importantly, the TRFM-LFIA strip was innovatively used for the quantitative detection of PdG in urine sample, and excellent recovery results were also obtained, ranging from 97.39% to 112.64%.Discussion: The TRFMLFIA strip possessed robust sensitivity and selectivity in the determination of PdG, indicating the great potential of being powerful tools in the biomedical and diagnosis region.

Published in Frontiers in Bioengineering and Biotechnology

ISSN: 2296-4185 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Technology: Chemical technology: Biotechnology
Website: http://www.frontiersin.org/bioengineering_and_biotechnology

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