Evaluation of quantitative parameters of parodontopathogenic and residential microflora in gingival sulcus biofilm in children and adolescents with dentoalveolar anomalies

Abstract

The method of bacteriologic investigation employing the anaerobic cultivation technique, as well as the molecular-genetic method based on polymerase chain reaction, were used to detect the major parodontopathogenic species and to conduct quantitative evaluation of parodontopathogenic and residential microflora in gingival sulcus biofilm in children and adolescents (aged 7-14) with dentoalveolar anomalies. It has been shown that a proper indicator best reflecting the intensity of morphological and functional disturbances in case of dentoalveolar anomalies in children and adolescents, is an increase of parodontopathogene genetic markers in gingival fluid (Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans, and Bacteroides forsythus).

Keywords

• молекулярно-генетический метод
• полимеразно-цепная реакция
• анаэробное культивирование
• зубочелюстные аномалии
• пародонтопатогенная микрофлора
• molecular-genetic method
• polymerase chain reaction
• anaerobic cultivation
• dentoalveolar anomalies
• parodontopathogenic microflora