Metabolic Engineering of Escherichia coli for the Production of l‑Homoserine

Abstract

l-Homoserine embodies significant functional properties as an amino acid of utmost importance, showcasing remarkable utility within the industrial realm. As synthetic biology and biotechnology continue to advance, the synthesis of l-homoserine through microbial fermentation emerges as a compelling and eco-conscious approach. This Review summarized the recent progress in systematic metabolic engineering strategies for improving l-homoserine production in Escherichia coli, including blocking the competing and degrading pathways, strengthening the key enzymes and precursors, and genetic modification of transport systems. We discussed and compared these systematic metabolism strategies, which have laid a solid foundation for the microbial industrial production of l-homoserine.