Frontiers in Plant Science (Mar 2023)
Identification of a pluripotency-inducing small compound, PLU, that induces callus formation via Heat Shock Protein 90-mediated activation of auxin signaling
- Yuki Nakashima,
- Yuki Nakashima,
- Yuka Kobayashi,
- Yuka Kobayashi,
- Mizuki Murao,
- Mizuki Murao,
- Rika Kato,
- Rika Kato,
- Hitoshi Endo,
- Asuka Higo,
- Asuka Higo,
- Rie Iwasaki,
- Mikiko Kojima,
- Yumiko Takebayashi,
- Ayato Sato,
- Mika Nomoto,
- Mika Nomoto,
- Hitoshi Sakakibara,
- Hitoshi Sakakibara,
- Yasuomi Tada,
- Yasuomi Tada,
- Kenichiro Itami,
- Kenichiro Itami,
- Seisuke Kimura,
- Seisuke Kimura,
- Shinya Hagihara,
- Keiko U. Torii,
- Keiko U. Torii,
- Naoyuki Uchida,
- Naoyuki Uchida,
- Naoyuki Uchida
Affiliations
- Yuki Nakashima
- Center for Gene Research, Nagoya University, Nagoya, Japan
- Yuki Nakashima
- Graduate School of Science, Nagoya University, Nagoya, Japan
- Yuka Kobayashi
- Center for Gene Research, Nagoya University, Nagoya, Japan
- Yuka Kobayashi
- School of Science, Nagoya University, Nagoya, Japan
- Mizuki Murao
- Center for Gene Research, Nagoya University, Nagoya, Japan
- Mizuki Murao
- Graduate School of Science, Nagoya University, Nagoya, Japan
- Rika Kato
- Graduate School of Science, Nagoya University, Nagoya, Japan
- Rika Kato
- Center for Sustainable Resource Science, RIKEN, Saitama, Japan
- Hitoshi Endo
- Institute of Transformative Bio-Molecules, Nagoya University, Nagoya, Japan
- Asuka Higo
- Center for Gene Research, Nagoya University, Nagoya, Japan
- Asuka Higo
- Institute for Advanced Research, Nagoya University, Nagoya, Japan
- Rie Iwasaki
- Institute of Transformative Bio-Molecules, Nagoya University, Nagoya, Japan
- Mikiko Kojima
- Center for Sustainable Resource Science, RIKEN, Yokohama, Japan
- Yumiko Takebayashi
- Center for Sustainable Resource Science, RIKEN, Yokohama, Japan
- Ayato Sato
- Institute of Transformative Bio-Molecules, Nagoya University, Nagoya, Japan
- Mika Nomoto
- Center for Gene Research, Nagoya University, Nagoya, Japan
- Mika Nomoto
- Graduate School of Science, Nagoya University, Nagoya, Japan
- Hitoshi Sakakibara
- Center for Sustainable Resource Science, RIKEN, Yokohama, Japan
- Hitoshi Sakakibara
- Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan
- Yasuomi Tada
- Center for Gene Research, Nagoya University, Nagoya, Japan
- Yasuomi Tada
- Graduate School of Science, Nagoya University, Nagoya, Japan
- Kenichiro Itami
- Graduate School of Science, Nagoya University, Nagoya, Japan
- Kenichiro Itami
- Institute of Transformative Bio-Molecules, Nagoya University, Nagoya, Japan
- Seisuke Kimura
- Department of Industrial Life Sciences, Faculty of Life Science, Kyoto Sangyo University, Kyoto, Japan
- Seisuke Kimura
- 0Center for Plant Sciences, Kyoto Sangyo University, Kyoto, Japan
- Shinya Hagihara
- Center for Sustainable Resource Science, RIKEN, Saitama, Japan
- Keiko U. Torii
- Institute of Transformative Bio-Molecules, Nagoya University, Nagoya, Japan
- Keiko U. Torii
- 1Howard Hughes Medical Institute and Department of Molecular Biosciences, University of Texas at Austin, Austin, TX, United States
- Naoyuki Uchida
- Center for Gene Research, Nagoya University, Nagoya, Japan
- Naoyuki Uchida
- Graduate School of Science, Nagoya University, Nagoya, Japan
- Naoyuki Uchida
- Institute of Transformative Bio-Molecules, Nagoya University, Nagoya, Japan
- DOI
- https://doi.org/10.3389/fpls.2023.1099587
- Journal volume & issue
-
Vol. 14
Abstract
Plants retain the ability to generate a pluripotent tissue called callus by dedifferentiating somatic cells. A pluripotent callus can also be artificially induced by culturing explants with hormone mixtures of auxin and cytokinin, and an entire body can then be regenerated from the callus. Here we identified a pluripotency-inducing small compound, PLU, that induces the formation of callus with tissue regeneration potency without the external application of either auxin or cytokinin. The PLU-induced callus expressed several marker genes related to pluripotency acquisition via lateral root initiation processes. PLU-induced callus formation required activation of the auxin signaling pathway though the amount of active auxin was reduced by PLU treatment. RNA-seq analysis and subsequent experiments revealed that Heat Shock Protein 90 (HSP90) mediates a significant part of the PLU-initiated early events. We also showed that HSP90-dependent induction of TRANSPORT INHIBITOR RESPONSE 1, an auxin receptor gene, is required for the callus formation by PLU. Collectively, this study provides a new tool for manipulating and investigating the induction of plant pluripotency from a different angle from the conventional method with the external application of hormone mixtures.
Keywords
