Chemometric Profiling and Bioactivity of Verbena (<i>Aloysia</i> <i>citrodora</i>) Methanolic Extract from Four Localities in Tunisia
Sonia Tammar,
Nidhal Salem,
Wissem Aidi Wannes,
Hajer Limam,
Soumaya Bourgou,
Nedia Fares,
Sarra Dakhlaoui,
Majdi Hammami,
Saber Khammassi,
Giovanni Del Re,
Kamel Hessini,
Kamel Msaada
Affiliations
Sonia Tammar
Laboratory of Aromatic and Medicinal Plants, Biotechnology Center in Borj-Cedria Technopol, BP. 901, Hammam-Lif 2050, Tunisia
Nidhal Salem
Laboratory of Bioactive Substances, Biotechnology Center in Borj-Cedria Technopol, BP. 901, Hammam-Lif 2050, Tunisia
Wissem Aidi Wannes
Laboratory of Aromatic and Medicinal Plants, Biotechnology Center in Borj-Cedria Technopol, BP. 901, Hammam-Lif 2050, Tunisia
Hajer Limam
Laboratory of Aromatic and Medicinal Plants, Biotechnology Center in Borj-Cedria Technopol, BP. 901, Hammam-Lif 2050, Tunisia
Soumaya Bourgou
Laboratory of Aromatic and Medicinal Plants, Biotechnology Center in Borj-Cedria Technopol, BP. 901, Hammam-Lif 2050, Tunisia
Nedia Fares
Laboratory of Bioactive Substances, Biotechnology Center in Borj-Cedria Technopol, BP. 901, Hammam-Lif 2050, Tunisia
Sarra Dakhlaoui
Laboratory of Aromatic and Medicinal Plants, Biotechnology Center in Borj-Cedria Technopol, BP. 901, Hammam-Lif 2050, Tunisia
Majdi Hammami
Laboratory of Aromatic and Medicinal Plants, Biotechnology Center in Borj-Cedria Technopol, BP. 901, Hammam-Lif 2050, Tunisia
Saber Khammassi
Laboratory of Aromatic and Medicinal Plants, Biotechnology Center in Borj-Cedria Technopol, BP. 901, Hammam-Lif 2050, Tunisia
Giovanni Del Re
Dipartimento di Ingegneria Industriale e dell’ Informazione e di Economia, Università dell’Aquila, Piazzale Ernesto Pontieri, Monteluco di Roio, 67100 L’Aquila, Italy
Kamel Hessini
Department of Biology, College of Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
Kamel Msaada
Laboratory of Aromatic and Medicinal Plants, Biotechnology Center in Borj-Cedria Technopol, BP. 901, Hammam-Lif 2050, Tunisia
This research aimed to study the chemical composition of Aloysia citrodora methanolic extract and its biological activities as an antioxidant, and its antibacterial, antifungal and anti-inflammatory activities based on four bioclimatic collection stages. The contents of total phenols, total flavonoids and total tannins were determined. Nine phenolic compounds were identified by LC-DAD–ESI-MS/MS. The major compound was acteoside, a phenylpropanoid which represented about 80% of the methanolic fraction in the various regions. The antioxidant activities of different locations were measured by different analytical assays, such as DPPH, ABTS and iron reducing power. The results showed that phenolic compounds and antioxidant activities varied with climatic and environmental factors. Moreover, there was a significant dependency between regions and biological activities. The use of a principal component analysis showed that there was a close relationship among phenylpropanoids, phenolic compounds and the studied biological activities.
