Memorias do Instituto Oswaldo Cruz (Mar 1992)

Detection of cytomegalovirus in urine of HIV-infected patients by DNA-DNA hybridization comparison with virus isolation, immunofluorescence and immunoperoxidase

  • Angel Valdivia,
  • Miguel Marrero,
  • Maritza Alvarez,
  • Mayra Mune,
  • Odalys Valdes,
  • German Roges

DOI
https://doi.org/10.1590/S0074-02761992000100016
Journal volume & issue
Vol. 87, no. 1
pp. 99 – 102

Abstract

Read online

Immunofluorescence and immunoperoxidase test directed against early viral antigens, and DNA-DNA hybridization were compared with viral isolation for their abilities to detect Cytomegalovirus (CVM) in the urine of 89 HIV infected patients. From the 100 urine samples collected, 70 were found positive by at least one method. Considering viral isolation as the "gold standard" technique, immunofluorescence and immunoperoxidase had a sensitivity of 92.3% and88% respectively, with a specificity in both cases of 95%. DNA-DNA hybridization showed a sensitivity of 90% but with lower (60%) specificity. All of the three assays were effective in detecting CVM from urine and the technical advantage of each is discussed.

Keywords