Influence of liposomal transfection of apolipoprotein E3 gene on neurologic and cognitive deficits dynamics at experimental traumatic brain injury

Abstract

The aim of study was to estimate gene therapy influence, that promotes synthesis induction of 3 apolipoprotein E (apoE3) in damaged brain tissue, on motor and memory dysfunction and learning disorders after experimental traumatic brain injury (TBI). Heavy TBI in rats was inflicted under overall anesthesia by free falling from1.5 mof load weighting450 g. The mixture of DOTAP liposome and 25 µg of plasmid vector pCMV•SPORT6 with cDNA of APOE3 gene was infused intraventricularly using ALZET osmotic pump. Posttraumatic neurologic deficits was estimated according to the scale of motor dysfunction at the first day of experiment before TBI infliction and from 1st to 7th day after trauma. Cognitive functions (spatial memory and learning) were tested in Morris water maze during 7–10 days after injury. The obtained results testified that cationic liposome-mediated APOE3 gene transfer caused quicker regress of neurologic and cognitive disorders after experimental TBI.