An Improved Enzyme-Linked Focus Formation Assay Revealed Baloxavir Acid as a Potential Antiviral Therapeutic Against Hantavirus Infection

Chuantao Ye; Chuantao Ye; Dan Wang; He Liu; Hongwei Ma; Yangchao Dong; Min Yao; Yuan Wang; Hui Zhang; Liang Zhang; Linfeng Cheng; Zhikai Xu; Yingfeng Lei; Fanglin Zhang; Wei Ye

doi:10.3389/fphar.2019.01203

Frontiers in Pharmacology (Oct 2019)

An Improved Enzyme-Linked Focus Formation Assay Revealed Baloxavir Acid as a Potential Antiviral Therapeutic Against Hantavirus Infection

Chuantao Ye,
Chuantao Ye,
Dan Wang,
He Liu,
Hongwei Ma,
Yangchao Dong,
Min Yao,
Yuan Wang,
Hui Zhang,
Liang Zhang,
Linfeng Cheng,
Zhikai Xu,
Yingfeng Lei,
Fanglin Zhang,
Wei Ye

Affiliations

Chuantao Ye: Department of Microbiology, School of Preclinical Medicine, Fourth Military Medical University, Xi’an, China
Chuantao Ye: Department of Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi’an, China
Dan Wang: School of Pharmaceutical Science, Shanxi Medical University, Taiyuan, China
He Liu: Department of Microbiology, School of Preclinical Medicine, Fourth Military Medical University, Xi’an, China
Hongwei Ma: Department of Microbiology, School of Preclinical Medicine, Fourth Military Medical University, Xi’an, China
Yangchao Dong: Department of Microbiology, School of Preclinical Medicine, Fourth Military Medical University, Xi’an, China
Min Yao: Department of Microbiology, School of Preclinical Medicine, Fourth Military Medical University, Xi’an, China
Yuan Wang: Department of Microbiology, School of Preclinical Medicine, Fourth Military Medical University, Xi’an, China
Hui Zhang: Department of Microbiology, School of Preclinical Medicine, Fourth Military Medical University, Xi’an, China
Liang Zhang: Department of Microbiology, School of Preclinical Medicine, Fourth Military Medical University, Xi’an, China
Linfeng Cheng: Department of Microbiology, School of Preclinical Medicine, Fourth Military Medical University, Xi’an, China
Zhikai Xu: Department of Microbiology, School of Preclinical Medicine, Fourth Military Medical University, Xi’an, China
Yingfeng Lei: Department of Microbiology, School of Preclinical Medicine, Fourth Military Medical University, Xi’an, China
Fanglin Zhang: Department of Microbiology, School of Preclinical Medicine, Fourth Military Medical University, Xi’an, China
Wei Ye: Department of Microbiology, School of Preclinical Medicine, Fourth Military Medical University, Xi’an, China

DOI: https://doi.org/10.3389/fphar.2019.01203
Journal volume & issue: Vol. 10

Abstract

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Hantaviruses, etiologic pathogens responsible for two severe human diseases, exist in areas ranging from Eurasia to America and remain global public health concerns. Conventionally, plaque formation assays have been used for hantavirus titering. However, hantaviruses replicate slowly within cells and produce minimal cytopathic effects, making this technique difficult to master. The improved enzyme-linked immunosorbent assay-based antigen detection method is easier to perform but is still time consuming. Here, we established an enzyme-linked focus formation assay (FFA) for Hantaan virus titering that is twice as fast as traditional assays. Moreover, using this method, we evaluated the effects of favipiravir (T-705) and another influenza virus drug, baloxavir acid (BXA), on hantavirus replication. We found that the endonuclease inhibitor BXA exerted similar anti-hantavirus effects as T-705. Overall, we developed a time-saving method for hantavirus titering and suggest BXA as a potential treatment choice for hantavirus-exposed individuals.

Published in Frontiers in Pharmacology

ISSN: 1663-9812 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: http://journal.frontiersin.org/journals/pharmacology

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