PROPAGAÇÃO "IN VITRO" DA CULTURA DO ABACAXIZEIRO ORNAMENTAL (ANANAS LUCIDUS MILLER)

Abstract

The in vitro propagation can provide the production of uniform plants, with high quality and free of diseases, and allow fast and genetically reliable multiplication of new cultivars and hybrids. In this context, the present work had the aim at developing a protocol for the in vitro propagation of Ananas lucidus Miller. Two experiments have been carried through, the first on the effect of BAP on nodal multiplication, an the second on plantlet acclimatization. For the multiplication it was used a completely randomized factorial design with 10 repetitions, and 2x5 factors, composed by two consistences, solid and liquid, and five BAP dosages (0,0; 1,0; 2,0; 3,0; 4,0) mg L-1. For the acclimatization experiment it was used a randomized blocks design with 5 treatments, 5 repetitions and 5 plants in each experimental unit, it being tested different substrata (coconut fiber, compost of plant residues, vermiculite, coconut fiber + compost, and vermiculite + compost). The number of sprouts (NB) produced in the liquid medium in the 2,0 mg. L-1 BAP dosage treatment was greater than in the 0,0 mg L-1 dosage, at a 5% probability level, and similar to the 1,0; 3,0 e 4,0 mg.L-1 dosages. It was observed that in the liquid media treatments there was a higher proliferation of sprouts than in the semi-solid media (11,3 and 9,0 respectively). The acclimatization survival index was of 96% for all treatments.