Nature Communications (Oct 2018)
Precisely measured protein lifetimes in the mouse brain reveal differences across tissues and subcellular fractions
- Eugenio F. Fornasiero,
- Sunit Mandad,
- Hanna Wildhagen,
- Mihai Alevra,
- Burkhard Rammner,
- Sarva Keihani,
- Felipe Opazo,
- Inga Urban,
- Till Ischebeck,
- M. Sadman Sakib,
- Maryam K. Fard,
- Koray Kirli,
- Tonatiuh Pena Centeno,
- Ramon O. Vidal,
- Raza-Ur Rahman,
- Eva Benito,
- André Fischer,
- Sven Dennerlein,
- Peter Rehling,
- Ivo Feussner,
- Stefan Bonn,
- Mikael Simons,
- Henning Urlaub,
- Silvio O. Rizzoli
Affiliations
- Eugenio F. Fornasiero
- Department of Neuro- and Sensory Physiology, University Medical Center Göttingen, Cluster of Excellence Nanoscale Microscopy and Molecular Physiology of the Brain
- Sunit Mandad
- Department of Neuro- and Sensory Physiology, University Medical Center Göttingen, Cluster of Excellence Nanoscale Microscopy and Molecular Physiology of the Brain
- Hanna Wildhagen
- Department of Neuro- and Sensory Physiology, University Medical Center Göttingen, Cluster of Excellence Nanoscale Microscopy and Molecular Physiology of the Brain
- Mihai Alevra
- Department of Neuro- and Sensory Physiology, University Medical Center Göttingen, Cluster of Excellence Nanoscale Microscopy and Molecular Physiology of the Brain
- Burkhard Rammner
- Department of Neuro- and Sensory Physiology, University Medical Center Göttingen, Cluster of Excellence Nanoscale Microscopy and Molecular Physiology of the Brain
- Sarva Keihani
- Department of Neuro- and Sensory Physiology, University Medical Center Göttingen, Cluster of Excellence Nanoscale Microscopy and Molecular Physiology of the Brain
- Felipe Opazo
- Department of Neuro- and Sensory Physiology, University Medical Center Göttingen, Cluster of Excellence Nanoscale Microscopy and Molecular Physiology of the Brain
- Inga Urban
- Genes and Behavior Department, Max Planck Institute of Biophysical Chemistry
- Till Ischebeck
- Department of Plant Biochemistry, Albrecht-von-Haller-Institute, Georg-August-University
- M. Sadman Sakib
- Laboratory of Epigenetics in Neurodegenerative Diseases, German Center for Neurodegenerative Diseases (DZNE)
- Maryam K. Fard
- German Center for Neurodegenerative Disease (DZNE)
- Koray Kirli
- Department of Cellular Logistics, Max Planck Institute for Biophysical Chemistry
- Tonatiuh Pena Centeno
- Laboratory of Computational Systems Biology, German Center for Neurodegenerative Diseases (DZNE)
- Ramon O. Vidal
- Laboratory of Computational Systems Biology, German Center for Neurodegenerative Diseases (DZNE)
- Raza-Ur Rahman
- Laboratory of Computational Systems Biology, German Center for Neurodegenerative Diseases (DZNE)
- Eva Benito
- Laboratory of Epigenetics in Neurodegenerative Diseases, German Center for Neurodegenerative Diseases (DZNE)
- André Fischer
- Laboratory of Epigenetics in Neurodegenerative Diseases, German Center for Neurodegenerative Diseases (DZNE)
- Sven Dennerlein
- Department of Cellular Biochemistry, University Medical Center Göttingen
- Peter Rehling
- Department of Cellular Biochemistry, University Medical Center Göttingen
- Ivo Feussner
- Department of Plant Biochemistry, Albrecht-von-Haller-Institute, Georg-August-University
- Stefan Bonn
- Laboratory of Computational Systems Biology, German Center for Neurodegenerative Diseases (DZNE)
- Mikael Simons
- German Center for Neurodegenerative Disease (DZNE)
- Henning Urlaub
- Department of Clinical Chemistry, University Medical Center Göttingen
- Silvio O. Rizzoli
- Department of Neuro- and Sensory Physiology, University Medical Center Göttingen, Cluster of Excellence Nanoscale Microscopy and Molecular Physiology of the Brain
- DOI
- https://doi.org/10.1038/s41467-018-06519-0
- Journal volume & issue
-
Vol. 9,
no. 1
pp. 1 – 17
Abstract
Measuring precise protein turnover rates in animals is technically challenging at the proteomic level. Here, Fornasiero and colleagues use isotopic labeling with mass spectrometry and mathematical modeling to accurately determine protein lifetimes in the mouse brain
