Transcriptomic and Anatomic Profiling Reveal Etiolation Promotes Adventitious Rooting by Exogenous Application of 1-Naphthalene Acetic Acid in <i>Robinia pseudoacacia</i> L.

Muhammad Zeeshan Munir; Saleem Ud Din; Muhammad Imran; Zijie Zhang; Tariq Pervaiz; Chao Han; Zaib Un Nisa; Ali Bakhsh; Muhammad Atif Muneer; Yuhan Sun; Yun Li

doi:10.3390/f12060789

Forests (Jun 2021)

Transcriptomic and Anatomic Profiling Reveal Etiolation Promotes Adventitious Rooting by Exogenous Application of 1-Naphthalene Acetic Acid in <i>Robinia pseudoacacia</i> L.

Muhammad Zeeshan Munir,
Saleem Ud Din,
Muhammad Imran,
Zijie Zhang,
Tariq Pervaiz,
Chao Han,
Zaib Un Nisa,
Ali Bakhsh,
Muhammad Atif Muneer,
Yuhan Sun,
Yun Li

Affiliations

Muhammad Zeeshan Munir: Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, Robinia pseudoacacia Engineering Technology Research Center of National Forestry and Grassland Administration, College of Biological Science and Technology, Beijing Forestry University, Beijing 100083, China
Saleem Ud Din: Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, Robinia pseudoacacia Engineering Technology Research Center of National Forestry and Grassland Administration, College of Biological Science and Technology, Beijing Forestry University, Beijing 100083, China
Muhammad Imran: State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
Zijie Zhang: Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, Robinia pseudoacacia Engineering Technology Research Center of National Forestry and Grassland Administration, College of Biological Science and Technology, Beijing Forestry University, Beijing 100083, China
Tariq Pervaiz: Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing 100083, China
Chao Han: Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, Robinia pseudoacacia Engineering Technology Research Center of National Forestry and Grassland Administration, College of Biological Science and Technology, Beijing Forestry University, Beijing 100083, China
Zaib Un Nisa: College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China
Ali Bakhsh: Department of Plant Breeding and Genetics, Ghazi University, Dera Ghazi Khan 32200, Pakistan
Muhammad Atif Muneer: International Magnesium Institute, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Yuhan Sun: Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, Robinia pseudoacacia Engineering Technology Research Center of National Forestry and Grassland Administration, College of Biological Science and Technology, Beijing Forestry University, Beijing 100083, China
Yun Li: Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, Robinia pseudoacacia Engineering Technology Research Center of National Forestry and Grassland Administration, College of Biological Science and Technology, Beijing Forestry University, Beijing 100083, China

DOI: https://doi.org/10.3390/f12060789
Journal volume & issue: Vol. 12, no. 6
p. 789

Abstract

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The process of etiolation contributes significantly to vegetative propagation and root formation of woody plants. However, the molecular interaction pattern of different factors for etiolated adventitious root development in woody plants remains unclear. In the present study, we explored the changes at different etiolation stages of adventitious root formation in Robinia pseudoacacia. Histological and transcriptomic analyses were performed for the etiolated lower portion of hypocotyls to ascertain the adventitious root responses. We found that the dark-treated hypocotyls formed roots earlier than the control. Exogenous application of NAA (0.3 mg/L) stimulated the expressions of about 310 genes. Among these, 155 were upregulated and 155 were downregulated. Moreover, differentially expressed genes (DEGs) were significantly enriched in multiple pathways, including the biosynthesis of secondary metabolites, metabolic pathway, plant hormone signal transduction, starch and sucrose metabolism, phenylpropanoid biosynthesis, and carbon metabolism. These pathways could play a significant role during adventitious root formation in etiolated hypocotyls. The findings of this study can provide novel insights and a foundation for further studies to elucidate the connection between etiolation and adventitious root formation in woody plants.

Published in Forests

ISSN: 1999-4907 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Botany: Plant ecology
Website: http://www.mdpi.com/journal/forests/

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