Вестник Московского государственного областного университета: Серия: Естественные науки (2017-11-01)

MARKER SYNTHESIS FOR IMMUNOFLUORESCENT ANALYSIS:N1-(p-ISOTHIOCYANATOBENZYL)-DIETHYLENETRIAMINE-N1, N2, N3, N3-TETRAACETIC ACID

  • Пугачев Дмитрий Евгеньевич,
  • Осин Николай Сергеевич,
  • Васильев Николай Валентинович

DOI
https://doi.org/10.18384/2310-7189-2017-3-51-60
Journal volume & issue
no. 3
pp. 51 – 60

Abstract

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A modified scheme for preparation of a widely used reagent for immunofluorescence analysis, an isothiocyanate derivative of diethylenetriamine tetraacetic acid, is proposed. Certain stages of the compound preparation are optimized and the product yield is significantly increased (up to 70%). The developed scheme allows one not to apply an additional stage of the isothiocyanate derivative purification using preparative chromatography.