MARKER SYNTHESIS FOR IMMUNOFLUORESCENT ANALYSIS:N1-(p-ISOTHIOCYANATOBENZYL)-DIETHYLENETRIAMINE-N1, N2, N3, N3-TETRAACETIC ACID

Вестник Московского государственного областного университета: Серия: Естественные науки. 2017;(3):51-60 DOI 10.18384/2310-7189-2017-3-51-60

 

Journal Homepage

Journal Title: Вестник Московского государственного областного университета: Серия: Естественные науки

ISSN: 2072-8352 (Print); 2310-7189 (Online)

Publisher: Moscow Region State University Editorial Office

Society/Institution: Moscow Region State University

LCC Subject Category: Science

Country of publisher: Russian Federation

Language of fulltext: Russian

Full-text formats available: PDF

 

AUTHORS


Пугачев Дмитрий Евгеньевич

Осин Николай Сергеевич

Васильев Николай Валентинович (Московский государственный областной университет)

EDITORIAL INFORMATION

Double blind peer review

Editorial Board

Instructions for authors

Time From Submission to Publication: 10 weeks

 

Abstract | Full Text

A modified scheme for preparation of a widely used reagent for immunofluorescence analysis, an isothiocyanate derivative of diethylenetriamine tetraacetic acid, is proposed. Certain stages of the compound preparation are optimized and the product yield is significantly increased (up to 70%). The developed scheme allows one not to apply an additional stage of the isothiocyanate derivative purification using preparative chromatography.