Structure- and computational-aided engineering of an oxidase to produce isoeugenol from a lignin-derived compound

Yiming Guo; Laura Alvigini; Milos Trajkovic; Lur Alonso-Cotchico; Emanuele Monza; Simone Savino; Ivana Marić; Andrea Mattevi; Marco W. Fraaije

doi:10.1038/s41467-022-34912-3

Nature Communications (Nov 2022)

Structure- and computational-aided engineering of an oxidase to produce isoeugenol from a lignin-derived compound

Yiming Guo,
Laura Alvigini,
Milos Trajkovic,
Lur Alonso-Cotchico,
Emanuele Monza,
Simone Savino,
Ivana Marić,
Andrea Mattevi,
Marco W. Fraaije

Affiliations

Yiming Guo: Molecular Enzymology Group, University of Groningen
Laura Alvigini: Department of Biology and Biotechnology “Lazzaro Spallanzani”, University of Pavia
Milos Trajkovic: Molecular Enzymology Group, University of Groningen
Lur Alonso-Cotchico: Zymvol Biomodeling S.L.
Emanuele Monza: Zymvol Biomodeling S.L.
Simone Savino: Molecular Enzymology Group, University of Groningen
Ivana Marić: Molecular Enzymology Group, University of Groningen
Andrea Mattevi: Department of Biology and Biotechnology “Lazzaro Spallanzani”, University of Pavia
Marco W. Fraaije: Molecular Enzymology Group, University of Groningen

DOI: https://doi.org/10.1038/s41467-022-34912-3
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 12

Abstract

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Lignin can be depolymerized into 4-alkylphenols by chemical means. Here the authors show a three-step computational-assisted enzyme engineering process to generate a biocatalyst for the conversion of lignin-derived 4-n-propylguaiacol into isoeugenol, a valuable compound.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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