Evaluation of a novel liquid stabilised peste des petits ruminants vaccine: Safety and immunogenic efficacy in sheep and goats in the field in Jordan
Fraser Crofts,
Ahmad Al-Majali,
David Gerring,
Simon Gubbins,
Matthew Tully,
Hayley Hicks,
Dana Campbell,
Jeremy Salt,
Steve Wilson,
Lizzie Chesang,
Kristin Stuke,
Claudia Cordel,
Satya Parida,
Carrie Batten
Affiliations
Fraser Crofts
The Pirbright Institute, Ash Road, Pirbright, Surrey GU24 0NF, United Kingdom
Ahmad Al-Majali
Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid 22110, Jordan; Subregional Office for the Gulf Cooperation Council States and Yemen, Food and Agriculture Organization of the United Nations (FAO), Abu Dhabi 62072, United Arab Emirates
David Gerring
Arecor Therapeutics PLC, Chesterford Research Park, Little Chesterford, Saffron Walden CB10 1XL, United Kingdom
Simon Gubbins
The Pirbright Institute, Ash Road, Pirbright, Surrey GU24 0NF, United Kingdom
Matthew Tully
The Pirbright Institute, Ash Road, Pirbright, Surrey GU24 0NF, United Kingdom
Hayley Hicks
The Pirbright Institute, Ash Road, Pirbright, Surrey GU24 0NF, United Kingdom
Dana Campbell
Dana Campbell Consultants Ltd, 15 Justice Park, Oxton, Lauderdale TD2 6NZ, United Kingdom
Jeremy Salt
GALVmed, Doherty Building, Pentlands Science Park, Bush Loan, Edinburgh EH26 0PZ, United Kingdom
Steve Wilson
GALVmed, International Livestock Research Institute (ILRI), Swing One, Naivasha Road, Nairobi, Kenya; GALVmed, Doherty Building, Pentlands Science Park, Bush Loan, Edinburgh EH26 0PZ, United Kingdom
Lizzie Chesang
GALVmed, International Livestock Research Institute (ILRI), Swing One, Naivasha Road, Nairobi, Kenya
Kristin Stuke
GALVmed, International Livestock Research Institute (ILRI), Swing One, Naivasha Road, Nairobi, Kenya
Claudia Cordel
GALVmed, International Livestock Research Institute (ILRI), Swing One, Naivasha Road, Nairobi, Kenya
Satya Parida
The Pirbright Institute, Ash Road, Pirbright, Surrey GU24 0NF, United Kingdom; Food and Agriculture Organization of the United Nations (FAO), Viale delle Terme di Caracalla, 00153 Rome, Italy; Corresponding authors.
Carrie Batten
The Pirbright Institute, Ash Road, Pirbright, Surrey GU24 0NF, United Kingdom; Corresponding authors.
A novel liquid stabiliser was tested with the Nigeria 75/1 Peste des Petit Ruminants (PPR) vaccine over two field studies carried out in sheep and goats. PPR seronegative sheep and goats were selected from farms surrounding Amman, Jordan and were vaccinated with either a stabilised liquid PPR vaccine that had been formulated 3 months prior to use and stored at 2–8 °C or a reconstituted lyophilised PPRV vaccine reconstituted on the day of vaccination. Sera were taken immediately before vaccination and at approximately 1.5, 3 and 6 months following vaccination, then subsequently tested using IDVet ID Screen® PPR competition ELISA and Serum Neutralisation tests to determine the presence of PPRV anti-N antibodies and neutralising antibodies, respectively. It was observed that the liquid-stabilised vaccine was able to provide comparable antibody responses in both species to those induced by the lyophilized vaccine. The ability to store liquid stabilised PPRV vaccine for field use would positively impact PPRV eradication efforts.