Frontiers in Plant Science (Jun 2015)
Use of 7cm immobilized pH gradient strips for the determination of clinically relevant proteins in wheat grain extract
Abstract
The aim of the work was to test relatively simple proteomics approach based on phenol extraction, two-dimensional electrophoresis (2-DE) in combination with 7cm immobilized pH gradient strips for the determination of clinically relevant proteins in wheat grain. Using this approach, 157 2-DE spots were quantified in biological triplicate, out of which 53 were identified by MALDI-TOF/TOF mass spectrometry. Clinically relevant proteins associated with celiac disease, wheat dependent exercise induced anaphylaxis, baker´s asthma, and food allergy, were detected in 24 2-DE spots. The comparison with recent quantitative study suggested that gel-based and gel-free proteomics approaches are complementary for the detection and quantification of clinically relevant proteins in wheat grain.
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