Integration of miRNA-lncRNA-mRNA profiles in liver tissue from EpCAM knockout mice

Lei Zili; Lei Yuting; Chen Guibin; Liu Shaomin; Liu Wanwan; Huang Li; Yang Lanxiang; Wu Huijuan; Yang Yanhong

doi:10.2298/ABS211207001L

Archives of Biological Sciences (Jan 2022)

Integration of miRNA-lncRNA-mRNA profiles in liver tissue from EpCAM knockout mice

Lei Zili,
Lei Yuting,
Chen Guibin,
Liu Shaomin,
Liu Wanwan,
Huang Li,
Yang Lanxiang,
Wu Huijuan,
Yang Yanhong

Affiliations

Lei Zili: Guangdong Metabolic Diseases Research Center of Integrated Chinese and Western Medicine; Guangdong TCM Key Laboratory for Metabolic Diseases; Key Laboratory of Glucolipid Metabolic Disorder, Ministry of Education of China; Institute of Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, P.R. China
Lei Yuting: Guangdong Metabolic Diseases Research Center of Integrated Chinese and Western Medicine; Guangdong TCM Key Laboratory for Metabolic Diseases; Key Laboratory of Glucolipid Metabolic Disorder, Ministry of Education of China; Institute of Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, P.R. China
Chen Guibin: Guangdong Metabolic Diseases Research Center of Integrated Chinese and Western Medicine; Guangdong TCM Key Laboratory for Metabolic Diseases; Key Laboratory of Glucolipid Metabolic Disorder, Ministry of Education of China; Institute of Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, P.R. China
Liu Shaomin: Guangdong Metabolic Diseases Research Center of Integrated Chinese and Western Medicine; Guangdong TCM Key Laboratory for Metabolic Diseases; Key Laboratory of Glucolipid Metabolic Disorder, Ministry of Education of China; Institute of Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, P.R. China + School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, Guangzhou, P.R. China
Liu Wanwan: Guangdong Metabolic Diseases Research Center of Integrated Chinese and Western Medicine; Guangdong TCM Key Laboratory for Metabolic Diseases; Key Laboratory of Glucolipid Metabolic Disorder, Ministry of Education of China; Institute of Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, P.R. China
Huang Li: Guangdong Metabolic Diseases Research Center of Integrated Chinese and Western Medicine; Guangdong TCM Key Laboratory for Metabolic Diseases; Key Laboratory of Glucolipid Metabolic Disorder, Ministry of Education of China; Institute of Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, P.R. China + School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, Guangzhou, P.R. China
Yang Lanxiang: Guangdong Metabolic Diseases Research Center of Integrated Chinese and Western Medicine; Guangdong TCM Key Laboratory for Metabolic Diseases; Key Laboratory of Glucolipid Metabolic Disorder, Ministry of Education of China; Institute of Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, P.R. China + School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, Guangzhou, P.R. China
Wu Huijuan: Guangdong Metabolic Diseases Research Center of Integrated Chinese and Western Medicine; Guangdong TCM Key Laboratory for Metabolic Diseases; Key Laboratory of Glucolipid Metabolic Disorder, Ministry of Education of China; Institute of Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, P.R. China + School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, Guangzhou, P.R. China
Yang Yanhong: First Affiliated Hospital (School of Clinical Medicine), Guangdong Pharmaceutical University, Guangzhou, P.R. China

DOI: https://doi.org/10.2298/ABS211207001L
Journal volume & issue: Vol. 74, no. 1
pp. 25 – 39

Abstract

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The epithelial cell adhesion molecule (EpCAM) is highly expressed in the liver during development and diseases. However, its role in the development and pathology of liver remains to be explored. The liver tissues of EpCAM-/- and wildtype (WT) mice at P0 stage were used for RNA sequencing. The differently expressed miRNAs, lncRNAs and mRNAs were selected and confirmed by qPCR. The expression of metabolism-related gene SET domain bifurcated 2 (Setdb2) was significantly increased in the liver of EpCAM-/- mice; the triglyceride (TG) and total cholesterol (TC) levels in the liver were also markedly decreased in EpCAM-/- mice. The microRNA (miRNA)-long noncoding RNA (lncRNA)-mRNA regulatory networks indicated that EpCAM may play important roles in glucose and lipid metabolism of the liver during development and in disease. The comprehensive miRNA, lncRNA and mRNA expression profiles in the developing liver of EpCAM-/- mice established here might help to elucidate functions and mechanisms of EpCAM during development and in diseases of the liver.

Published in Archives of Biological Sciences

ISSN: 0354-4664 (Print); 1821-4339 (Online)
Publisher: University of Belgrade, University of Novi Sad
Country of publisher: Serbia
LCC subjects: Science: Biology (General)
Website: http://www.serbiosoc.org.rs/arch/index.php

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