Frontiers in Marine Science (Aug 2022)
Optimizing cryopreservation of sea cucumber (Apostichopus japonicus) sperm using a programmable freezer and computer-assisted sperm analysis
- Shuai Xu,
- Shuai Xu,
- Shuai Xu,
- Shuai Xu,
- Shuai Xu,
- Shuai Xu,
- Shilin Liu,
- Shilin Liu,
- Shilin Liu,
- Shilin Liu,
- Shilin Liu,
- Shilin Liu,
- Jingchun Sun,
- Jingchun Sun,
- Jingchun Sun,
- Jingchun Sun,
- Jingchun Sun,
- Libin Zhang,
- Libin Zhang,
- Libin Zhang,
- Libin Zhang,
- Libin Zhang,
- Libin Zhang,
- Chenggang Lin,
- Chenggang Lin,
- Chenggang Lin,
- Chenggang Lin,
- Chenggang Lin,
- Chenggang Lin,
- Lina Sun,
- Lina Sun,
- Lina Sun,
- Lina Sun,
- Lina Sun,
- Lina Sun,
- Lili Xing,
- Lili Xing,
- Lili Xing,
- Lili Xing,
- Lili Xing,
- Lili Xing,
- Chunxi Jiang,
- Chunxi Jiang,
- Chunxi Jiang,
- Chunxi Jiang,
- Chunxi Jiang,
- Chunxi Jiang,
- Hongsheng Yang,
- Hongsheng Yang,
- Hongsheng Yang,
- Hongsheng Yang,
- Hongsheng Yang,
- Hongsheng Yang,
- Hongsheng Yang
Affiliations
- Shuai Xu
- Chinese Academy of Sciences (CAS) Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China
- Shuai Xu
- Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China
- Shuai Xu
- Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, China
- Shuai Xu
- Chinese Academy of Sciences (CAS) Engineering Laboratory for Marine Ranching, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China
- Shuai Xu
- University of Chinese Academy of Sciences, Beijing, China
- Shuai Xu
- Shandong Province Key Laboratory of Experimental Marine Biology, Qingdao, China
- Shilin Liu
- Chinese Academy of Sciences (CAS) Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China
- Shilin Liu
- Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China
- Shilin Liu
- Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, China
- Shilin Liu
- Chinese Academy of Sciences (CAS) Engineering Laboratory for Marine Ranching, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China
- Shilin Liu
- University of Chinese Academy of Sciences, Beijing, China
- Shilin Liu
- Shandong Province Key Laboratory of Experimental Marine Biology, Qingdao, China
- Jingchun Sun
- Chinese Academy of Sciences (CAS) Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China
- Jingchun Sun
- Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China
- Jingchun Sun
- Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, China
- Jingchun Sun
- Chinese Academy of Sciences (CAS) Engineering Laboratory for Marine Ranching, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China
- Jingchun Sun
- Shandong Province Key Laboratory of Experimental Marine Biology, Qingdao, China
- Libin Zhang
- Chinese Academy of Sciences (CAS) Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China
- Libin Zhang
- Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China
- Libin Zhang
- Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, China
- Libin Zhang
- Chinese Academy of Sciences (CAS) Engineering Laboratory for Marine Ranching, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China
- Libin Zhang
- University of Chinese Academy of Sciences, Beijing, China
- Libin Zhang
- Shandong Province Key Laboratory of Experimental Marine Biology, Qingdao, China
- Chenggang Lin
- Chinese Academy of Sciences (CAS) Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China
- Chenggang Lin
- Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China
- Chenggang Lin
- Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, China
- Chenggang Lin
- Chinese Academy of Sciences (CAS) Engineering Laboratory for Marine Ranching, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China
- Chenggang Lin
- University of Chinese Academy of Sciences, Beijing, China
- Chenggang Lin
- Shandong Province Key Laboratory of Experimental Marine Biology, Qingdao, China
- Lina Sun
- Chinese Academy of Sciences (CAS) Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China
- Lina Sun
- Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China
- Lina Sun
- Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, China
- Lina Sun
- Chinese Academy of Sciences (CAS) Engineering Laboratory for Marine Ranching, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China
- Lina Sun
- University of Chinese Academy of Sciences, Beijing, China
- Lina Sun
- Shandong Province Key Laboratory of Experimental Marine Biology, Qingdao, China
- Lili Xing
- Chinese Academy of Sciences (CAS) Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China
- Lili Xing
- Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China
- Lili Xing
- Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, China
- Lili Xing
- Chinese Academy of Sciences (CAS) Engineering Laboratory for Marine Ranching, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China
- Lili Xing
- University of Chinese Academy of Sciences, Beijing, China
- Lili Xing
- Shandong Province Key Laboratory of Experimental Marine Biology, Qingdao, China
- Chunxi Jiang
- Chinese Academy of Sciences (CAS) Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China
- Chunxi Jiang
- Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China
- Chunxi Jiang
- Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, China
- Chunxi Jiang
- Chinese Academy of Sciences (CAS) Engineering Laboratory for Marine Ranching, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China
- Chunxi Jiang
- University of Chinese Academy of Sciences, Beijing, China
- Chunxi Jiang
- Shandong Province Key Laboratory of Experimental Marine Biology, Qingdao, China
- Hongsheng Yang
- Chinese Academy of Sciences (CAS) Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China
- Hongsheng Yang
- Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China
- Hongsheng Yang
- Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, China
- Hongsheng Yang
- Chinese Academy of Sciences (CAS) Engineering Laboratory for Marine Ranching, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China
- Hongsheng Yang
- University of Chinese Academy of Sciences, Beijing, China
- Hongsheng Yang
- Shandong Province Key Laboratory of Experimental Marine Biology, Qingdao, China
- Hongsheng Yang
- The Innovation of Seed Design, Chinese Academy of Sciences, Wuhan, China
- DOI
- https://doi.org/10.3389/fmars.2022.917045
- Journal volume & issue
-
Vol. 9
Abstract
The sea cucumber Apostichopus japonicus has high nutritional, medicinal, and economic value. However, factors such as overexploitation, climate change, and environmental pollution have resulted in serious germplasm degradation in both farmed and wild A. japonicus, and it has been listed as endangered on the IUCN (International Union for Conservation of Nature) Red List of Threatened Species. Cryopreservation is an important method to protect germplasm resources and solve the problem of germplasm degradation. Using a programmable freezer and computer-aided sperm analysis, we comprehensively studied and screened the factors that affect the post-thaw motility of A. japonicus sperm during cryopreservation. Based on our results, we propose the following optimal cryopreservation procedure for A. japonicus sperm: cryo-diluent composition of 12.5% dimethyl sulfoxide and 0.1 mol/L glucose, with filter-sterilized (the filter mesh size: 0.45μm) natural seawater (NSW) as the extender; 1:5 mixing ratio of sperm and cryo-diluent; cooling rate and thawing temperatures of 10°C/min and 20°C, respectively. The post-thaw motility of sperm treated using the optimal procedure was > 65%, the fertilization rate (in the blastocyst stage) was nearly 80%, and the hatching rate (in the early auricularia larva stage) was > 65%. Additionally, frozen sperm that had been cryopreserved for 1 year retained a considerable post-thaw motility and fertilization rate compared to recently cryopreserved sperm. We detected obvious differences in sperm freezability among individual A. japonicus, and cryopreservation caused some damage to the sperm structure. In conclusion, our optimized procedure make large-scale cryopreservation of A. japonicus sperm possible, and our results provide valuable information that could be applied to research and conservation of A. japonicus.
Keywords