A digital microfluidic single-cell manipulation system optimized by extending-depth-of-field device

Qiushu Chen; Qi Meng; Yuzhe Liu; Xiangan Long; Yawei Kong; Longfang Yao; Liwen Chen; Chuanyong Wu; Kaiqin Chu; Lan Mi; Jiong Ma

doi:10.1142/S1793545822440060

Journal of Innovative Optical Health Sciences (May 2023)

A digital microfluidic single-cell manipulation system optimized by extending-depth-of-field device

Qiushu Chen,
Qi Meng,
Yuzhe Liu,
Xiangan Long,
Yawei Kong,
Longfang Yao,
Liwen Chen,
Chuanyong Wu,
Kaiqin Chu,
Lan Mi,
Jiong Ma

Affiliations

Qiushu Chen: Department of Optical Science and Engineering, Shanghai Engineering Research Center of Ultra-precision Optical Manufacturing, Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), School of Information Science and Technology, Fudan University, Shanghai 200433, P. R. China
Qi Meng: Department of Precision Machinery and Precision Instrumentation, University of Science and Technology of China, Hefei 230027, Anhui, P. R. China
Yuzhe Liu: Department of Optical Science and Engineering, Shanghai Engineering Research Center of Ultra-precision Optical Manufacturing, Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), School of Information Science and Technology, Fudan University, Shanghai 200433, P. R. China
Xiangan Long: Institute of Biomedical Engineering and Technology, Academy for Engineering and Technology, Fudan University, Shanghai 200433, P. R. China
Yawei Kong: Department of Optical Science and Engineering, Shanghai Engineering Research Center of Ultra-precision Optical Manufacturing, Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), School of Information Science and Technology, Fudan University, Shanghai 200433, P. R. China
Longfang Yao: Department of Optical Science and Engineering, Shanghai Engineering Research Center of Ultra-precision Optical Manufacturing, Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), School of Information Science and Technology, Fudan University, Shanghai 200433, P. R. China
Liwen Chen: Department of Optical Science and Engineering, Shanghai Engineering Research Center of Ultra-precision Optical Manufacturing, Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), School of Information Science and Technology, Fudan University, Shanghai 200433, P. R. China
Chuanyong Wu: Shanghai Hengxin BioTechnology, Ltd., 1688 North Guo Quan Rd, Bldg A8, Rm 801, Shanghai 200438, P. R. China
Kaiqin Chu: Department of Precision Machinery and Precision Instrumentation, University of Science and Technology of China, Hefei 230027, Anhui, P. R. China
Lan Mi: Department of Optical Science and Engineering, Shanghai Engineering Research Center of Ultra-precision Optical Manufacturing, Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), School of Information Science and Technology, Fudan University, Shanghai 200433, P. R. China
Jiong Ma: Department of Optical Science and Engineering, Shanghai Engineering Research Center of Ultra-precision Optical Manufacturing, Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), School of Information Science and Technology, Fudan University, Shanghai 200433, P. R. China

DOI: https://doi.org/10.1142/S1793545822440060
Journal volume & issue: Vol. 16, no. 03

Abstract

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Microfluidic systems have been widely utilized in high-throughput biology analysis, but the difficulties in liquid manipulation and cell cultivation limit its application. This work has developed a new digital microfluidic (DMF) system for on-demand droplet control. By adopting an extending-depth-of-field (EDoF) phase modulator to the optical system, the entire depth of the microfluidic channel can be covered in one image without any refocusing process, ensuring that 95% of the particles in the droplet are captured within three shots together with shaking processes. With this system, suspension droplets are generated and droplets containing only one yeast cell can be recognized, then each single cell is cultured in the array of the chip. By observing their growth in cell numbers and the green fluorescence protein (GFP) production via fluorescence imaging, the single cell with the highest production can be identified. The results have proved the heterogeneity of yeast cells, and showed that the combined system can be applied for rapid single-cell sorting, cultivation, and analysis.

Published in Journal of Innovative Optical Health Sciences

ISSN: 1793-5458 (Print); 1793-7205 (Online)
Publisher: World Scientific Publishing
Country of publisher: Singapore
LCC subjects: Technology; Science: Physics: Optics. Light
Website: http://www.worldscientific.com/worldscinet/jiohs

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