In vitro propagation of lnula royleana DC

Abstract

A micropropagation method, through axillary shoot proliferation, was elaborated for Inula royleana DC. (Asteraceae), a medicinal plant native of Himalaya. Primary explants (cotyledonary node explants) and secondary explants (node explants of in vitro regenerated shoots) of the plant, inoculated on MS medium supplemented with 0.1 μM NAA and 5.0 μM kinetin, regenerated 3.4 ± 1.2 and 5.1 ± 1.9 axillary shoots per explant, respectively. The regenerated shoots were easily rooting and adapting to growth in soil.

Keywords

• Inula royleana
• axillary shoots
• micropropagation
• Asteraceae