Three New Clerodane Diterpenes from Polyalthia longifolia var. pendula
Tung-Ho Wu,
Yung-Yi Cheng,
Chao-Jung Chen,
Lean-Teik Ng,
Li-Chen Chou,
Li-Jiau Huang,
Yung-Husan Chen,
Sheng-Chu Kuo,
Mohamed El-Shazly,
Yang-Chang Wu,
Fang-Rong Chang,
Chih-Chuang Liaw
Affiliations
Tung-Ho Wu
Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807, Taiwan
Yung-Yi Cheng
Graduate Institute of Pharmaceutical Chemistry, China Medical University, Taichung 404, Taiwan
Chao-Jung Chen
Proteomics Core Laboratory, Department of Medical Research, China Medical University Hospital, Taichung 404, Taiwan
Lean-Teik Ng
Department of Agricultural Chemistry, National Taiwan University, Taipei 106, Taiwan
Li-Chen Chou
Graduate Institute of Pharmaceutical Chemistry, China Medical University, Taichung 404, Taiwan
Li-Jiau Huang
Graduate Institute of Pharmaceutical Chemistry, China Medical University, Taichung 404, Taiwan
Yung-Husan Chen
National Museum of Marine Biology and Aquarium, Pingtung 944, Taiwan
Sheng-Chu Kuo
Graduate Institute of Pharmaceutical Chemistry, China Medical University, Taichung 404, Taiwan
Mohamed El-Shazly
Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, Ain-Shams University, Organization of African Unity Street, Abassia, Cairo 11566, Egypt
Yang-Chang Wu
Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807, Taiwan
Fang-Rong Chang
Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807, Taiwan
Chih-Chuang Liaw
Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804, Taiwan
Three new clerodane diterpenes, (4→2)-abeo-cleroda-2,13E-dien-2,14-dioic acid (1), (4→2)-abeo-2,13-diformyl-cleroda-2,13E-dien-14-oic acid (2), and 16(R&S)- methoxycleroda-4(18),13-dien-15,16-olide (3), were isolated from the unripe fruit of Polyalthia longifolia var. pendula (Annonaceae) together with five known compounds (4–8). The structures of all isolates were determined by spectroscopic analysis. The anti-inflammatory activity of the isolates was evaluated by testing their inhibitory effect on NO production in LPS-stimulated RAW 264.7 macrophages. Among the isolated compounds, 16-hydroxycleroda-3,13-dien-15,16-olide (6) and 16-oxocleroda-3,13-dien-15-oic acid (7) showed promising NO inhibitory activity at 10 µg/mL, with 81.1% and 86.3%, inhibition, respectively.
