Rhodamine B induces oxidative stress and cervical epithelial cell proliferation in the uterus

Abstract

This study aimed to investigate the effect of Rhodamine B exposure on oxidative stress and cervical epithelial cells proliferation in the uterus. Twenty eight female Wistar albino rats were divided into four groups (n = 7 each): one control (untreated) group; and three Rhodamine B groups at several doses (4.5, 9, 18 mg/200 g body weight/day) for 36 days. Colometric analysis of malondialdehyde (MDA) level as a marker of lipid peroxidation and histological analysis of the cervical epithelial cells proliferation was performed. The MDA levels and proliferation of epithelial cells were significantly higher in all Rhodamine B groups compared to control group (P < 0.05). The MDA levels were increased in a dose-dependent manner in the Rhodamine B groups. Moreover, the proliferation of epithelial cells was also increased by Rhodamine B in a dose-dependent manner. In conclusion, subchronic Rhodamine B administration induces lipid peroxidation and cervical epithelial cells proliferation in a dose dependent manner.

Keywords

• Cervical epithelial cell of the uterus
• Cell proliferation
• Xenobiotic
• Dye
• Oxidative stress