Fabrication, microstructure and hemostatic activity of Cu-Zn manganite nanoparticles

Abstract

Copper subbed zinc manganite Zn(1-x)CuxMn2O4 (x = 0.05,0.15,0.25,0.35,0.45,0.55) (BCZMO) NPs were prepared by sol–gel co-precipitation technique at surrounding temperature. BCZMO NPs were analyzed by XRD, SEMfor microstructure characterizations. XRD study confirms the tetragon structure of BCZMO NPs and effect of copper on ZMO NPs. SEM micrographs supports our findings. The BCZMO NPs interfered in blood coagulation and exhibited pro-coagulant property. BCZMO NPs decreased the coagulation time of citrated plasma from control 4.31 min to 1.35 min. In addition, the BCZMO NPs aggregated platelets up to 80 % at a concentration of 600 µg while the agonist epinephrine prompted platelet collection of around 90 % at 10 mM concentration.No kind of hemolytic activity in RBC cells shown by these BCZMO-NPs insisting their non-toxic nature. These BCZMO-NPs exhibited pro-coagulant effect with their interference in blood coagulation cascade and initiation in platelet aggregation. Thus, BCZMO NPs can be a potential candidate in biomedical field as hemostatic agents.

Keywords

• Cu-Zn manganites
• Pro-coagulant
• Platelet aggregation
• Non-toxic
• Hemostatic