Nature Communications (May 2019)
Structure and immunogenicity of a stabilized HIV-1 envelope trimer based on a group-M consensus sequence
- Kwinten Sliepen,
- Byung Woo Han,
- Ilja Bontjer,
- Petra Mooij,
- Fernando Garces,
- Anna-Janina Behrens,
- Kimmo Rantalainen,
- Sonu Kumar,
- Anita Sarkar,
- Philip J. M. Brouwer,
- Yuanzi Hua,
- Monica Tolazzi,
- Edith Schermer,
- Jonathan L. Torres,
- Gabriel Ozorowski,
- Patricia van der Woude,
- Alba Torrents de la Peña,
- Mariëlle J. van Breemen,
- Juan Miguel Camacho-Sánchez,
- Judith A. Burger,
- Max Medina-Ramírez,
- Nuria González,
- Jose Alcami,
- Celia LaBranche,
- Gabriella Scarlatti,
- Marit J. van Gils,
- Max Crispin,
- David C. Montefiori,
- Andrew B. Ward,
- Gerrit Koopman,
- John P. Moore,
- Robin J. Shattock,
- Willy M. Bogers,
- Ian A. Wilson,
- Rogier W. Sanders
Affiliations
- Kwinten Sliepen
- Department of Medical Microbiology, Amsterdam Infection & Immunity Institute, Amsterdam UMC, University of Amsterdam
- Byung Woo Han
- Department of Integrative Structural and Computational Biology, Scripps CHAVI-ID, IAVI Neutralizing Antibody Center and Collaboration for AIDS Vaccine Discovery (CAVD), The Scripps Research Institute
- Ilja Bontjer
- Department of Medical Microbiology, Amsterdam Infection & Immunity Institute, Amsterdam UMC, University of Amsterdam
- Petra Mooij
- Department of Virology, Biomedical Primate Research Centre
- Fernando Garces
- Department of Integrative Structural and Computational Biology, Scripps CHAVI-ID, IAVI Neutralizing Antibody Center and Collaboration for AIDS Vaccine Discovery (CAVD), The Scripps Research Institute
- Anna-Janina Behrens
- Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford
- Kimmo Rantalainen
- Department of Integrative Structural and Computational Biology, Scripps CHAVI-ID, IAVI Neutralizing Antibody Center and Collaboration for AIDS Vaccine Discovery (CAVD), The Scripps Research Institute
- Sonu Kumar
- Department of Integrative Structural and Computational Biology, Scripps CHAVI-ID, IAVI Neutralizing Antibody Center and Collaboration for AIDS Vaccine Discovery (CAVD), The Scripps Research Institute
- Anita Sarkar
- Department of Integrative Structural and Computational Biology, Scripps CHAVI-ID, IAVI Neutralizing Antibody Center and Collaboration for AIDS Vaccine Discovery (CAVD), The Scripps Research Institute
- Philip J. M. Brouwer
- Department of Medical Microbiology, Amsterdam Infection & Immunity Institute, Amsterdam UMC, University of Amsterdam
- Yuanzi Hua
- Department of Integrative Structural and Computational Biology, Scripps CHAVI-ID, IAVI Neutralizing Antibody Center and Collaboration for AIDS Vaccine Discovery (CAVD), The Scripps Research Institute
- Monica Tolazzi
- Viral Evolution and Transmission Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute
- Edith Schermer
- Department of Medical Microbiology, Amsterdam Infection & Immunity Institute, Amsterdam UMC, University of Amsterdam
- Jonathan L. Torres
- Department of Integrative Structural and Computational Biology, Scripps CHAVI-ID, IAVI Neutralizing Antibody Center and Collaboration for AIDS Vaccine Discovery (CAVD), The Scripps Research Institute
- Gabriel Ozorowski
- Department of Integrative Structural and Computational Biology, Scripps CHAVI-ID, IAVI Neutralizing Antibody Center and Collaboration for AIDS Vaccine Discovery (CAVD), The Scripps Research Institute
- Patricia van der Woude
- Department of Medical Microbiology, Amsterdam Infection & Immunity Institute, Amsterdam UMC, University of Amsterdam
- Alba Torrents de la Peña
- Department of Medical Microbiology, Amsterdam Infection & Immunity Institute, Amsterdam UMC, University of Amsterdam
- Mariëlle J. van Breemen
- Department of Medical Microbiology, Amsterdam Infection & Immunity Institute, Amsterdam UMC, University of Amsterdam
- Juan Miguel Camacho-Sánchez
- Department of Medical Microbiology, Amsterdam Infection & Immunity Institute, Amsterdam UMC, University of Amsterdam
- Judith A. Burger
- Department of Medical Microbiology, Amsterdam Infection & Immunity Institute, Amsterdam UMC, University of Amsterdam
- Max Medina-Ramírez
- Department of Medical Microbiology, Amsterdam Infection & Immunity Institute, Amsterdam UMC, University of Amsterdam
- Nuria González
- AIDS Immunopathology Unit, Instituto de Salud Carlos III
- Jose Alcami
- AIDS Immunopathology Unit, Instituto de Salud Carlos III
- Celia LaBranche
- Department of Surgery, Duke University Medical Center
- Gabriella Scarlatti
- Viral Evolution and Transmission Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute
- Marit J. van Gils
- Department of Medical Microbiology, Amsterdam Infection & Immunity Institute, Amsterdam UMC, University of Amsterdam
- Max Crispin
- Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford
- David C. Montefiori
- Department of Surgery, Duke University Medical Center
- Andrew B. Ward
- Department of Integrative Structural and Computational Biology, Scripps CHAVI-ID, IAVI Neutralizing Antibody Center and Collaboration for AIDS Vaccine Discovery (CAVD), The Scripps Research Institute
- Gerrit Koopman
- Department of Virology, Biomedical Primate Research Centre
- John P. Moore
- Department of Microbiology and Immunology, Weill Medical College of Cornell University
- Robin J. Shattock
- Section of Virology, Division of Infectious Diseases, Department of Medicine, Imperial College London
- Willy M. Bogers
- Department of Virology, Biomedical Primate Research Centre
- Ian A. Wilson
- Department of Integrative Structural and Computational Biology, Scripps CHAVI-ID, IAVI Neutralizing Antibody Center and Collaboration for AIDS Vaccine Discovery (CAVD), The Scripps Research Institute
- Rogier W. Sanders
- Department of Medical Microbiology, Amsterdam Infection & Immunity Institute, Amsterdam UMC, University of Amsterdam
- DOI
- https://doi.org/10.1038/s41467-019-10262-5
- Journal volume & issue
-
Vol. 10,
no. 1
pp. 1 – 16
Abstract
Stabilized, native-like trimers of the HIV envelope protein, such as SOSIP trimers, are potential antigens for an HIV vaccine. Here, the authors generate a SOSIP trimer based on the consensus sequence of group M isolates, determine its structure and exposure of common epitopes, and show immunogenicity in rabbits and non-human primates.
