Immobilization of lipase in silica gel from rice husk ash and its activity assay to hydrolyze palm oil

Abstract

A free lipase is one of the biocatalysts used for industrial applications, especially to catalyze the hydrolysis of palm oil. However, it is unstable in an extreme condition so it is easy to denature. Immobilization of lipase improve the enzyme's stability since the cage of the immobilization matrix around the lipase can minimalize denaturation. Silica gel is the most chosen matrix because of its high thermal stability and inertness. Lipase was immobilized in silica gel extracted from rice husk ash. Silica gel was prepared in a sodium silicate solution. Sol-gel process occurred when phosphoric acid was added into the sodium silicate solution until it reached a pH of 7. The immobilization process was initiated by reacting lipase in Phosphate Buffer Solution (PBS) added to the sol solution to produce hydrogel. Hydrogel was got into the dry process to form xerogel. The activity assay was conducted in the hydrolysis reaction by titrimetric method. The immobilized lipase resulted had an immobilization percentage of 67.71% and reusability for 6 cycles.

Keywords

• hydrolysis
• immobilization
• lipase
• silica
• sol-gel